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稻米淀粉RVA谱特征值与直链淀粉、蛋白含量的相关性及QTL定位分析

张杰 郑蕾娜 蔡跃 尤小满 孔飞 汪国湘 燕海刚 金洁 王亮

中国水稻科学2017,Vol.31Issue(1):31-39,9.
中国水稻科学2017,Vol.31Issue(1):31-39,9.DOI:10.16819/j.1001-7216.2017.6046

稻米淀粉RVA谱特征值与直链淀粉、蛋白含量的相关性及QTL定位分析

Correlation Analysis and QTL Mapping for Starch RVA Profile Properties and Amylose and Protein Contents in Rice

张杰 1郑蕾娜 1蔡跃 1尤小满 1孔飞 1汪国湘 1燕海刚 1金洁 1王亮1

作者信息

  • 1. 南京农业大学 作物遗传与种质创新国家重点实验室/江苏省植物基因工程技术研究中心,南京 210095
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摘要

Abstract

[Objective]The detection of new quantitative trait loci(QTLs)and the correlation analysis between different rice quality traits are used to dissect the genetic mechanism underlying rice quality and to breed high-quality rice varie-ties.[Method]The backcross inbred lines(BILs)of Sasanishiki× Habataki planted in two environments were used to detect additive QTLs for amylose content,protein content and RVA profiles.[Result]Phenotypic analysis showed the protein content of Habataki was higher than that of Sasanishiki while other rice quality parameters of Sasanishiki were generally higher than those of Habataki except the setback viscosity.A total of 42 additive QTLs were detected,and ten of them could be repeatedly detected in two environments,including qPC8 ,qAC4 ,qAC10 ,qPKV2 ,qPKV7 , qH PV7 ,qCPV1 ,qBDV4 ,qBDV7 and qSBV7 .Of them,qCPV1 ,qBDV4 ,qPKV7 ,qH PV7 and qAC10 had not been reported previously.Moreover,the environmental stability of 10 QTLs,was further confirmed across two differ-ent environments by chromosome segment substitution lines(CSSLs).[Conclusion]Significant correlations were ob-served between RVA profiles and protein or amylose contents,and many QTLs affecting different quality traits were co-localized.

关键词

水稻/稻米品质/数量性状基因座/回交重组自交系/染色体片段置换系

Key words

rice/grain quality/quantitative trait locus/backcross inbred lines/chromosome segment substitution lines

分类

生物科学

引用本文复制引用

张杰,郑蕾娜,蔡跃,尤小满,孔飞,汪国湘,燕海刚,金洁,王亮..稻米淀粉RVA谱特征值与直链淀粉、蛋白含量的相关性及QTL定位分析[J].中国水稻科学,2017,31(1):31-39,9.

基金项目

国家转基因生物新品种培育重大专项(2014ZX08001006) (2014ZX08001006)

国家自然科学基金资助项目(31301296) (31301296)

国家科技支撑计划资助项目(2013BAD01B02-16) (2013BAD01B02-16)

江苏省科技支撑计划资助项目(BE2014394). (BE2014394)

中国水稻科学

OA北大核心CSCDCSTPCD

1001-7216

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