中国水稻科学2017，Vol.31Issue(1)：40-49,10.DOI:10.16819/j.1001-7216.2017.6117
长江流域主要常规双季早籼稻的遗传相似性分析及指纹图谱构建
Analysis of Genetic Similarity and Construction of DNA Fingerprinting for Conventional Double Cropping Early Rice Varieties in the Yangtze River Basin
摘要
Abstract
[Objective]To provide a reference for rice variety identification and parents selection,we constructed fingerprints of conventional early rice varieties and analyzed its genetic similarity.[Method]Conventional early rice varieties registered at or above the provincial level since 2000 in the Yangtze River Basin were analyzed using 50 pairs of SSR primers with high polymorphism,good stability,and even distribution on 12 chromosomes of rice to construct SSR fingerprints of conventional early rice.Cluster analysis was conducted with NTSYS PC Version 2.10e software and UPGMA cluster analysis method.[Results]A total of 154 polymorphic bands were detected among 62 rice lines.Two to six alleles were detected for each marker with an average of 3.08.Primers polymorphism information content (PIC) value ranged from 0.03 to 0.75 ,with an average of 0.36.Cluster analysis with NTSYS PC Version 2.10e software showed that the genetic similarities ranged from 0.66 to 1 .00.The 62 varieties could be divided into two groups as varieties in Zhejiang Province and varieties outside the province at the similarity coefficient of 0.73 with small difference between groups.[Conclusion]Combination with the pedigree relationship,it was revealed that the genetic background of early rice varieties in this study was relatively narrow.关键词
早稻/系谱/DNA指纹图谱/聚类分析Key words
early rice/pedigree/DNA fingerprinting/clustering analysis分类
农业科技引用本文复制引用
戴冬青,张华丽,张萌,李西明,马良勇..长江流域主要常规双季早籼稻的遗传相似性分析及指纹图谱构建[J].中国水稻科学,2017,31(1):40-49,10.基金项目
国家863计划资助项目(214AA10A604-15) （214AA10A604-15）
国家自然科学基金资助项目(31501288) （31501288）
浙江省自然科学基金资助项目(LQ16C130002) （LQ16C130002）
浙江省高产优质多抗水稻新品种选育(9410计划)资助项目(2012C12901-2) （9410计划）
中国水稻研究所中央级公益性科研院所基本科研业务费专项(2014RG003-3). （2014RG003-3）
