中国水稻科学2017，Vol.31Issue(1)：105-110,6.DOI:10.16819/j.1001-7216.2017.6060
水稻抗性基因Pi对福建省稻瘟病菌优势菌群的抗性分析
Analysis on Resistance of Pi Genes to Predominant Races of Mangnaporthe oryzae in Fujian Province,China
摘要
Abstract
[Obiective]The rational utilization and deployment of the blast resistance in rice varieties are key factors that ensures the durability and effectiveness of resistance genes.To determine the resistance and utilization potential of 24 single major resistance genes in Fujian Province,[Method]we tested the physiological races and pathotypes of 347 single spore isolates of Magnaporthe oryzae which were isolated from susceptible rice cultivar "Lijiangxintuanheigu"from different rice growing areas within Fujian Province between 2012-2015 by using seven Chinese host differentials and six CO39 near-isogenic lines (CO39 NILs)respectively.We further performed an assay to monitor the resistance frequencies of 24 single resistance genes with these isolates.[Result]Subsequently,we observed that the seven Chinese host differentials clearly differentiated the 347 isolates into six distinct groups with 36 physiological races.The dominant groups were ZA,ZB and ZC,and the dominant physiological races were ZC15 ,ZD7 and ZB15 .In addition,we noticed that the six CO39 NILs differentiated the 347 isolates into 17 pathotypes with I34.1 as the dominating pathotypes. Particularly,the resistance spectrums associated with the 24 resistance gene were larger and ranged from 9.80 to 8991 % when inoculated with the 347 isolates.For the eight resistance genes Pi-k m ,Pi- 7(t),Pi- 9(t),Pi-k p ,Pi-k , Pi-k h ,Pi-z 5 and Pi-ta (1 ),their resistance spectrums were above 70.00%.[Conclusion]These results indicated that the eight resistance genes could be successfully deployed in Fujian Province,and further demonstrated that rice breed-ing approaches could be combined with multiple resistance genes in widely grown hybrids or cultivars.Furthermore, the recorded resistance spectrums of eight genes for dominant physiological races and pathotypes were above 69.00%and adequately proved that the use of dominant physiological races or pathotypes as a method for identifying resistance or resistance spectrums in rice cultivars was reliable.关键词
稻瘟病菌/生理小种/致病型/抗性基因/抗谱Key words
Magnaporthe oryzae/physiological race/pathotype/resistance gene/resistance spectrum分类
生物科学引用本文复制引用
阮宏椿,石妞妞,杜宜新,甘林,杨秀娟,代玉立,陈福如..水稻抗性基因Pi对福建省稻瘟病菌优势菌群的抗性分析[J].中国水稻科学,2017,31(1):105-110,6.基金项目
国家公益性行业(农业)科研专项(201203014) （农业）
福建省属公益类科研院所基本科研专项项目(2015R1024-1 ,2016R1023-11) （2015R1024-1 ,2016R1023-11）
福建省科技厅项目(2009N2005). （2009N2005）
