中国输血杂志2017，Vol.30Issue(1)：77-79,3.DOI:10.13303/j.cjbt.issn.1004-549x.2017.01.026
血液筛查核酸检测结果无效原因分析
Analysis of the result invalidation in nucleic acid amplification technology for blood screening
摘要
Abstract
Objective To analyze the cause of the result invalidation in Cobas s201 nucleic acid detection systems (NAT) for blood screening.Methods The number and types of invalid results in NAT from October 2014 to December 2015 were counted.When combined with the amplification graphs,alarm information and test results,the relationship among detection time,detection systems (system A,B and C) and reagent lots (Lot 1,Lot 2,Lot 3,Lot 4,Lot 5 and Lot 6) and result invalidation were analyzed.Also,the distribution and composition of various types of invalid results (interior standard amplification invalidation,negative or positive control amplification invalidation,amplification graph abnormality and error codes with flags) were analyzed.Results The total rate of result invalidation in the lab was 0.21％ (47/22 284).Among the types of invalidation,the proportion of interior standard amplification invalidation was the most (51.06％).There were significant differences in the rates of the result invalidation in different months (x2=21.600,P＜0.05).There were significant differences in the rates of the result invalidation among detection systems (x2 =28.76,P＜0.05) and among different reagent lots (x2 =35.737,P＜0.05).The rates of the result invalidation in the detection systems C were higher,and were the highest in reagent Lot 2.Conclusion There was some correlation between different detection systems,different reagent lots and result invalidation.Monitoring the result invalidation closely will help to continuously monitor the nucleic acid detection performance.关键词
核酸检测/结果无效率/Cobas s201/原因分析Key words
nucleic acid amplification technology/rate of the result invalidation/Cobass201/analysis分类
医药卫生引用本文复制引用
陈少彬,何子毅,陈庆恺,余霖,王庆,邹文涛..血液筛查核酸检测结果无效原因分析[J].中国输血杂志,2017,30(1):77-79,3.基金项目
东莞市医疗卫生类科技计划一般项目(2015105101245) （2015105101245）
