中国实验动物学报2017，Vol.25Issue(1)：1-7,7.DOI:10.3969/j.issn.1005-4847.2017.01.001
水温改变对斑马鱼脊髓损伤修复的影响
Effect of water temperature on the recovery of spinal cord injury in zebrafish
摘要
Abstract
Objective Using zebrafish to analyze the effect of water temperature on the recovery of spinal cord in-jury. To detect the cell proliferation and changes of gene expression at the injury site during the process of recovery. Meth-ods Surgical operation was performed to induce spinal cord injury ( SCI) on adult fish. Water at a series of temperature was applied to culture the fish. Swimming ability was adopted to observe the recovery of spinal cord injury following surger?y. Vibration sections and immunohistochemistry were performed to observe the cell number post SCI at different stages. The changes of gdnf and nos gene expression were determined by real?time PCR. Results The water temperature changes from 28℃ to 32℃ did not affect the swimming ability of non?injured and sham?injured fish ( P>0. 05 ) . The swimming ability recovered mostly in 8 weeks post spinal cord injury. At 32℃, the swimming ability recovered faster than at 28℃ or at 30℃(P<0. 05). The cell proliferation increased obviously following spinal cord injury (P<0. 05). The proliferation of cells surrounding the spinal cord in jury was more extensive in SCI fishes incubated in 32℃ water than in 28℃ or 30℃ water ( P<0. 05). Real?time PCR assay showed that gdnf was up?regulated in all groups post SCI at 24 h, and 7 and 14 days (P<0. 05). The nos expression was up?regulated in all groups following SCI in 24 h (P<0. 05) and 7 days. There was no sig?nificant difference between the SCI group and sham?injury group (P<0. 05), while after 14 days, the expression of nos was reduced in the SCI group compared with the sham?injury group (P<0. 05). Conclusions A slight increase of incu?bating water temperature can accelerate the recovery of spinal cord injury in zebrafish.关键词
脊髓损伤/水疗/斑马鱼/gdnf/nosKey words
Spinal cord injury/Aquatic therapy/Zebrafish/gdnf/nos分类
生物科学引用本文复制引用
李强,郑苏林,冯瑜菲,杨振国,张晶晶..水温改变对斑马鱼脊髓损伤修复的影响[J].中国实验动物学报,2017,25(1):1-7,7.基金项目
国家自然科学基金(31370824) （31370824）
广东省"培养高层次人才特殊支持计划"专项基金(粤人才办〔2015〕8号) （粤人才办〔2015〕8号）
广东省高等学校优秀青年教师培养计划〔YQ2015081〕 （）
湛江市非资助科技攻关项目. （）
