中国实验动物学报2017，Vol.25Issue(1)：8-13,6.DOI:10.3969/j.issn.1005-4847.2017.01.002
利用表达谱芯片数据筛选不同表达丰度的内参基因
Screening of reference genes of different abundance using gene expression microarrays
摘要
Abstract
Objective Based on the data detected using gene expression microarray, to select multiple reference genes and use them to quantify transcriptome genes of different abundance in the mouse liver tissue. Methods To detect global transcriptome genes in the mouse liver tissues using gene expression microarray. All the genes were sorted into differ-ent groups according to their expression level, followed by coefficient of variation ( CV) analysis. Real?time quantitative polymerase chain reaction ( qPCR) and geNorm software were used to further identify the reference genes. Results The expression levels of over 60,000 genes were obtained from microarray screening, and divided them into low, moderate and high expression groups. Finally six reference genes were screened, i. e. Casp2 and Lrrc14 as low abundance, Nrd1 and Tr?pc4ap as moderate abundance, and Atp5a1 and Clu as high abundance genes. Conclusions The six reference genes de?rived from microarray data can be used to accurately quantify the global transcriptome genes with various expression levels.关键词
内参基因/表达谱芯片/实时荧光定量PCR/geNorm软件Key words
Reference genes/Gene expression microarrays/Quantitative real-time PCR/geNorm software/Mouse/Liver分类
生物科学引用本文复制引用
胡世贤,陆佳涛,徐福意,晁天柱,周梁良,李凯,周宇荀,肖君华..利用表达谱芯片数据筛选不同表达丰度的内参基因[J].中国实验动物学报,2017,25(1):8-13,6.基金项目
国家自然科学基金面上项目(编号:31371257) （编号:31371257）
上海市科委关键项目(编号:12140900404,13140900300,15140900500). （编号:12140900404,13140900300,15140900500）
