中国实验动物学报2017，Vol.25Issue(1)：36-42,7.DOI:10.3969/j.issn.1005-4847.2017.01.007
肺腺癌脑转移动物模型的生物发光成像和SPECT/CT的比较
Comparison between bioluminescence imaging and SPECT/CT of mouse models of brain metastasis from lung adenocarcinoma
摘要
Abstract
Objective To establish a mouse model of lung adenocarcinoma brain metastasis with human luc+-PC?9 cells stably expressing luciferase and to compare the evaluation values of bioluminescence imaging and18 F?FDG ( 18 F?flu?orodeoxyglucose) SPECT/CT in these models. Methods Suspension of luc+?PC?9 cells was injected into the left ventri?cle of BALB/c nude mice to establish a mouse model of brain metastasis from lung cancer. Bioluminescence imaging and18 F?FDG SPECT/CT were used to evaluate the metastasis of tumors as compared with HE?staining pathology as a golden standard. Results The success rate of brain metastases was 85% through injecting luc+?PC?9 cells into the left ventricle. The number of tumor cells was positively related to the intensity of light, with a linear correlation (R2 =0. 96). Fluores?cence was observed in the brain, spine and femur by bioluminescence imaging, and the metastases were confirmed by H&E pathological examination. 18 F?FDG SPECT/CT observed abnormal density collective foci in the spine or femur but not in the brain. Conclusions Injection of tumor cell suspension into the mouse left ventricle is a good method to establish a brain metastasis of lung cancer. Bioluminescence has a higher sensitivity and specificity in detecting brain metastasis and bone metastasis, with advantages of real?time, dynamical and non?invasive detection of tumor metastasis growth. 18 F?FDG SPECT/CT does not have superiority in detection of brain metastases but is suitable for detecting bone metastasis.关键词
肺癌/脑转移/骨转移/动物模型/生物发光成像/单光子发射计算机断层成像术Key words
Lung cancer/Brain metastasis/Bone metastasis/Animal model/Bioluminescence imaging/Single-photon emission computed tomography/computed tomography分类
生物科学引用本文复制引用
陈愉生,陈正伟,俞梅娥,涂洵崴,李鸿茹,林凌,林如辉..肺腺癌脑转移动物模型的生物发光成像和SPECT/CT的比较[J].中国实验动物学报,2017,25(1):36-42,7.基金项目
国家卫生计生委科研基金-福建省卫生教育联合攻关计划项目(WKJ?FJ?17) （WKJ?FJ?17）
福建省立医院优秀青年医师项目(2014YNQN03) （2014YNQN03）
福建省自然科学基金(2015J01376). （2015J01376）
