中国实验动物学报2017，Vol.25Issue(1)：74-78,84,6.DOI:10.3969/j.issn.1005-4847.2017.01.014
高脂环境下雄激素缺乏对小型猪内脏脂肪蓄积和炎症基因表达的影响
Effects of androgen deficiency on visceral fat accumulation and inflammatory gene expression in miniature pigs fed a high-fat diet
摘要
Abstract
Objective The aim of this study was to explore the effect of androgen deficiency on serum hormone levels, visceral fat accumulation and inflammatory gene expression in miniature pigs fed a high-fat diet ( HFD) . Methods Sexually mature male Chinese Wuzhishan miniature pigs were divided into three groups ( animals/group) as follows:intact male pigs ( SHAM) , castrated male pigs ( CAS) and castrated male pigs plus testosterone treatment ( CAS+T) . The pigs were fed a HFD diet for 12 weeks. Serum levels of testosterone and leptin were measured and visceral fat were dissected and weighted. qRT?PCR was performed to determine the mRNA expression levels of lipogenic, lipolysis and inflammation relat?ed genes. Results (1) Serum testosterone levels were significantly decreased but serum leptin levels were significantly in?creased in the castrated pigs. These effects were recovered after testosterone treatment. ( 2 ) Visceral fat percentage was significantly increased in the castrated pigs, and testosterone treatment reduced the increased visceral fat in the castrated pigs. (3) Castration and testosterone treatment had no significant effects on the expression levels of lipogenic genes (FAS and ACC) and lipolysis genes (HSL and ATGL) in pigs fed a HFD. (5) Castration significantly induced the expressions of inflammatory genes including Leptin, CD68, CCL16, CCL23 and SAA, and testosterone treatment recovered the expres?sions of the above genes in the castrated pigs. Conclusions Castration?induced testosterone deficiency promotes visceral fat accumulation and upregulates the expression levels of inflammatory genes in miniature pigs fed a HFD. Moreover, tes?tosterone treatment ameliorates castration?induced visceral fat accumulation and inflammatory response in HFD?fed pigs.关键词
雄激素/小型猪/肥胖/脂肪沉积/炎症基因Key words
Testosterone/Miniature pigs/Obesity/Fat accumulation/Inflammatory gene分类
生物科学引用本文复制引用
蔡兆伟,凌云,蔡月琴,朱科燕,陈诚..高脂环境下雄激素缺乏对小型猪内脏脂肪蓄积和炎症基因表达的影响[J].中国实验动物学报,2017,25(1):74-78,84,6.基金项目
浙江省公益性技术应用研究(实验动物)计划项目(编号:2014C37010) （实验动物）
国家自然科学基金项目(编号:31200921). （编号:31200921）
