中国比较医学杂志2017，Vol.27Issue(1)：5-10,6.DOI:10.3969.j.issn.1671-7856.2017.01.002
高脂饮食喂养建立高脂血症模型的验证及规律探讨
Validation Research And Regulation Exploration Of High Fat-introduced Hyperlipidemia Model In Rat
摘要
Abstract
Objective To investigate the feasibility of high fat-introduced hyperlipeidemia model in male SD rat and study the time rule of molding .Methods 30 Male adult rats of SD Strain bred in the animal house of the institute were divided into 3 groups after 1 week adaptation , group 1:control group, normal diet;group 2:model 1 group, high fat high cholesterol diet;group 3:model 2 group, high fat high cholesterol diet .The period of experiment was 8 weeks.Food and water intake were measured everyday and body weight were measured every four days .Blood were collected by orbital venous at the end of fourth ,sixth,eighth week to test their serum lipid level .At the end of experiment ,animals were killed to collect liver and aorta tissue for HE stain .Results Compared with control group ,the food intake of model 1 was higher and model 2 was significant lower , water intake of model 2 was significant lower , the ratio of liver/weight of two model groups were significant heavier ,and weight of model groups were higher .High fat diet significantly increased TC levels of model groups at the end of fourth ,sixth week.The level of LDL-c in model 1 group were higher and the HDL-c were lower compared with control group .HE stain showed the livers of control group were regular ,arrangements of the liver cells were trim, dyeing present uniformity .The two model groups showed a large range of hepatocyte fatty change ,a few liver blood sinus were in congestion and infiltrated with inflammatory cells .Aorta HE stain showed no significant change among 3 groups.Conclusions The method of high fat-introduced hyperlipeidemia model in male SD rat is feasible and the model turned out to present hypercholesterolemia with severe fatty liver .On the other hand,levers of serum lipid increased within an increase—inter-adjustment—increase state .In the process of modeling ,how to overcome the symptom of anorexia and the state of cholesterol inter-adjustment in animals is the key to successfully establish hyperlipeidemia model .关键词
高脂血症/动物模型/高脂饮食Key words
Hyperlipeidemia/Animal models/High fat diet分类
医药卫生引用本文复制引用
王燕萍,彭丹虹,刘晓琪,谢蓉,李先涛..高脂饮食喂养建立高脂血症模型的验证及规律探讨[J].中国比较医学杂志,2017,27(1):5-10,6.基金项目
国家重点基础研究发展计划(2014CB542901). （2014CB542901）
