中国比较医学杂志2017，Vol.27Issue(1)：11-15,21,6.DOI:10.3969.j.issn.1671-7856.2017.01.003
Smad3对非小细胞肺癌A549细胞和宫颈癌HeLa细胞迁移的影响
Effect of Smad3 on cell migration of A549 and HeLa cells
摘要
Abstract
Objective To investigate the effect of Smad3 on cell migration of A549 and HeLa cells.Methods Primers for pCMV-Myc-Smad3 plasmid construction and siRNA targeting Smad 3 were designed and synthesized .pCMV-Myc-Smad3 plasmid was constructed with molecular cloning techniques .Overexpression of Smad 3 with Myc-tag or silencing of endogenous Smad3, and then scratch assay was used to detect the migration ability of A 549 and HeLa cells in vitro. Results pCMV-Myc-Smad3 plasmid was successfully constructed .Overexpression of Smad 3 significantly up-regulated the migration rate of A549 and HeLa cells.Conversely, in the same cells, silencing of endogenous Smad3 or treatment with Smad3 inhibitor, SIS3, down-regulated the migration rate .Conclusions Smad3 promotes cell migration of A549 and HeLa cells.关键词
Smad3/过表达载体/RNA干扰/SIS3/细胞迁移Key words
Smad3/Overexpression vector/RNAi/SIS3/Cell migration分类
医药卫生引用本文复制引用
韦荣飞,李梦媛,杨星九,朱瑞敏,徐大模,高苒..Smad3对非小细胞肺癌A549细胞和宫颈癌HeLa细胞迁移的影响[J].中国比较医学杂志,2017,27(1):11-15,21,6.基金项目
协和青年基金资助,中央高校基本科研业务费专项资金资助(3332016077) （3332016077）
中国医学科学院医学实验动物研究所基本科研业务费专项资助(2016ZX310033) （2016ZX310033）
北京市优秀人才培养项目(2015000020124G073). （2015000020124G073）
