中国比较医学杂志2017，Vol.27Issue(1)：22-26,5.DOI:10.3969.j.issn.1671-7856.2017.01.005
不同时间点不同应激方法致大鼠焦虑样行为的研究
Research of different methods of stress-induced anxiety-like behavior in rats at different time points
摘要
Abstract
Objective To compare the biological characteristics of several different anxiety rat models established by different methods of stress at different time points and provide experimental basis for the most appropriate modeling methods .Methods 60 rats were randomly divided into normal , empty bottle stress , chronic emotional stress ( CES ) group, restraint stress for 3h, 6h, and modeling respectively .In the experimental 7 d, 14 d, 21 d, elevated plus maze and fear condition system was used to test anxiety-like behavior in rats , open field test to study anxiety or depression-like behavior , forced swimming test was used to detect depression-like behavior in rats , and using the Elisa test kit to detect the contents of 5-HT, DA in the hippocampus in rats .Results Anxiety-like behavioral test results showed that rats in empty bottle stress, CES, 6 h restraint stress group started to have anxiety-like behavior since 14 d, then anxiety-like behavior was becoming increasingly apparent .Forced swimming test results showed that immobility time in 6 h restraint rats was significantly increased in the first 7 d(P <0.05).Meanwhile, compared with control group, hippocampal 5-HT, DA contents in empty bottle stress and CES rats increased significantly since 14 d.Conclusions Among several stress methods established anxiety model , anxiety-like behavior in 3 h restraint stress was not obvious; 6 h restraint stress exhibited a depression-like behavior in the forced swimming test might be due to prolonged stress .Empty bottle stress and CES can successfully establish the anxiety rat model , and the anxiety behavior of the rats have some differences . Corresponding model methods can be selected according to different experimental purposes .关键词
焦虑模型/空瓶应激/慢性情绪应激/慢性束缚应激Key words
Anxiety model/Empty bottle stress/Chronic emotional stress/Chronic restraint stress分类
医药卫生引用本文复制引用
赵洪庆,韩远山,杜青,杨琴,王宇红,杨蕙,孟盼..不同时间点不同应激方法致大鼠焦虑样行为的研究[J].中国比较医学杂志,2017,27(1):22-26,5.基金项目
湖南省科技厅重点项目(2015DK3003) （2015DK3003）
湖南省研究生创新课题(CX2016B371,2016CX14). （CX2016B371,2016CX14）
