中国比较医学杂志2017，Vol.27Issue(1)：43-48,6.DOI:10.3969.j.issn.1671-7856.2017.01.009
广东小鼠腺病毒血清学调查及病毒和抗体在人工感染小鼠体内消长规律研究
Serological Survey of Mad Infection Among Mice in Guangdong and Distribution and Serological Studies in Artificial Infection
摘要
Abstract
Objective To investigate the natural infection rate of Mouse Adenovirus ( Mad) in Guangdong province and to estimate the tissue distribution and serology changes of Mad in infected mice .Methods Serums of mice from general environment of Guangzhou in 2007 and 2015, and the SPF mice from12 supervision and 32 entrusted units of Guangdong province among 2013 ~2015 were detected by ELISA .Thirty-six 3 weeks old BALB/c mice were each intraperitoneally inoculated with 0.2 mL of Mad in 4.5×106 copies/μL concentration.The tissue samples, including heart, liver, spleen, lung, kidney, brain, stomach , cecum contents and serum were taken at 0 d before inoculation, and 3, 7, 10, 15, 18, 21, 30, 37, 44, 51, 60 d after inoculation (3 animals each time).Real-time fluorescent quantitative PCR (Q-PCR) method was used to detect the virus nucleic acid of tissue .In addition, the antibody against Mad was detected by ELISA .Results Antibody positive rate was 24.44%~84.15%and 0%among the general environment mice and the SPF mice , respectively , which is given priority to with Mad-2 K87 serotype strains .There were no clinical manifestations among all infected mice.The virus nucleic acid positive rate was 100%(3/3) in all tissue samples in 3 d and 7 d, which maintained a longest time to 60 d in spleen.Besides the liver, virus nucleic acid content were highest at 7 d in each tissue of infected mice, with spleen highest (5.5×105 copies/μL), heart was second at 3.4×105 copies/μL, cecum contents and stomach ranked third ( 2.6×105 copies/μL), followed by brain (0.8×105 copies/μL).The antibody could be successfully detected at 15 d after inoculated, hit the peak at 37 d and maintained a high level until 60 d.Conclusions Mad infection rate is low in SPF mice, high in general environment mice.Mad nucleic acid content is the highest and positive rate maintain the longest in spleen ,suggesting that Mad mainly replicate in spleen .And 7 d is the best pot for nucleic acid testing.Serum neutralizing antibody can be used as a monitoring index within 15~60 d after infection.关键词
小鼠腺病毒/血清学/调查/人工感染/体内分布Key words
Mouse adenovirus/Serology/Survey/Artificial infection/Distribution.引用本文复制引用
罗银珠,张钰,潘金春,王静,袁文,何丽芳,吴瑞可,黄碧洪,郭鹏举..广东小鼠腺病毒血清学调查及病毒和抗体在人工感染小鼠体内消长规律研究[J].中国比较医学杂志,2017,27(1):43-48,6.基金项目
广东省科技计划项目(2014B070706006). （2014B070706006）
