中国比较医学杂志2017，Vol.27Issue(1)：49-53,5.DOI:10.3969.j.issn.1671-7856.2017.01.010
补肾养血方对卵巢早衰大鼠中抗苗勒氏管激素的影响
Effect of bushenyangxue prescription on the levels of anti-mullerian hormone in rat with premature ovarian failure
摘要
Abstract
Objective Establish premature ovarian failure ( POF ) model in female Sprague Dawley by tripterygium wilfordii , and investigate the effect of bushenyangxue prescription on the levels of anti -mullerian hormone ( AMH) .Methods After POF model was established , rats were gave by gavage of different dosage of Bushenyangxue prescription for 30 days.The changes of histomorphology on rat ovarian tissue were observed by hematoxylin -eosin staining. Serum AMH concentraction , protein and mRNA expression of AMH were measure with ELISA , immunohistochemical staining and qRT-PCR, respectively .Results The follicle and corpus luteum were atrophied after tripterygium wilfordii challenge, which was improved after treatment with Bushenyangxue prescription .Serum AMH, protein and mRNA expression of AMH were decreased tripterygium wilfordii-treated rats; this decrease was inhibited after treatment with Bushenyangxue prescription .Conclusions Our study indicates that Bushenyangxue prescription could preserve the AMH levels of POF rats.These findings suggest that Bushenyangxue prescription may be a useful strategy to treat POF .关键词
补肾养血方/卵巢早衰/AMHKey words
Bushenyangxue prescription/Premature ovarian failure ( POF)/AMH分类
医药卫生引用本文复制引用
姬霞,傅金英,王冰玉,胡俊攀..补肾养血方对卵巢早衰大鼠中抗苗勒氏管激素的影响[J].中国比较医学杂志,2017,27(1):49-53,5.基金项目
河南省中医药管理局科研课题(2014ZY02013). （2014ZY02013）
