中国比较医学杂志2017，Vol.27Issue(2)：15-20,6.DOI:10.3969.j.issn.1671-7856.2017.02.003
小鼠脾脏间充质干细胞调节T淋巴细胞的增殖与活化
Modulatory effects of mouse spleen?derived mesenchymal stem cells on the proliferation and activation of T lymphocytes
摘要
Abstract
Objective To investigate the modulatory effects of mouse spleen?derived mesenchymal stem cells ( Sp?MSCs) on the proliferation and activation of T lymphocytes. Methods The mouse Sp?MSCs were isolated from mouse spleens by using explant tissue culture protocol and the cells were induced to differentiate into osteoblasts and adipocytes. The effects of Sp?MSCs on proliferation of activated T lymphocytes were determined by carboxyfluorescein succinimidyl ester (CFSE) staining assay. Furthermore, the effects of Sp?MSCs on lymphocyte transformation test ( LTT) and mixed lymphocytes reaction ( MLR ) were observed, respectively. In addition, the mRNA expression of T cell derived proinflammatory cytokines by Sp?MSCs was detected by quantitative PCR. Results The results of CFSE assay demonstrated that Sp?MSCs suppressed ConA and allogeneic lymphocyte?induced T cell proliferation. Moreover, the Sp?MSCs were capable of suppressing LLT and MLR. Additionally, the results of quantitative PCR revealed that Sp?MSCs significantly suppressed the T cells expressing interferon?γ, tumor necrosis factor?α and interleukine?17A. Conclusions Sp?MSCs are capable of suppressing the proliferation and activation of T lymphocytes as well as the expression of T cell?derived proinflammatory cytokines.关键词
脾脏间充质干细胞/T淋巴细胞/增殖/活化/炎性细胞因子Key words
Spleen⁃derived mesenchymal stem cells/T lymphocytes/Proliferation/Activation/Proinflammatory cytokines/Mouse分类
医药卫生引用本文复制引用
丁丽,朱恒,张海宏,杨洋,韩冬梅,王志东,郑晓丽,董磊,闫洪敏..小鼠脾脏间充质干细胞调节T淋巴细胞的增殖与活化[J].中国比较医学杂志,2017,27(2):15-20,6.基金项目
国家自然科学基金项目(81500083,81371945,81572159,81101342) （81500083,81371945,81572159,81101342）
空军总医院院级课题(KZ2014023) （KZ2014023）
北京市自然科学基金项目(7132133). （7132133）
