中国比较医学杂志2017，Vol.27Issue(2)：21-24,4.DOI:10.3969.j.issn.1671-7856.2017.02.004
裸小鼠胃癌原位移植模型的建立
Establishment of an orthotopic gastric cancer model in nude mice
摘要
Abstract
Objective To establish a new orthotopic gastric cancer model in nude mice. Methods The BALB/c nude mice were anesthesized by Matrx VIP 3000 type gas anesthesia systems. Orthotopic gastric cancer models were established by embedding method, thread?ligation method or gastric bursa method in the nude mice. The tumor growth after transplantation in living body was detected by a Caliper IVIS Kinetic small animal imaging system. Samples of the transplanted tumor tissues for histological examination were taken at 28 days after transplantation. Results The orthotopic gastric cancer model was successfully established by embedding method. Compared with the models prepared by other commonly used methods, it showed obvious advantages such as a high success rate of transplantation, simple operation, short time, less difficulty, and less direct contact of the implanted tumor tissue with other organs, etc. Conclusions Large amount of mouse models of orthotopic gastric cancer can be prepared rapidly by the embedding method, thus, provide a convenient tool for related research of gastric cancer in future.关键词
胃癌/原位移植/小动物活体成像/气体麻醉Key words
Gastric Cancer/Orthotopic transplantation/Fluorescence imaging/in vivo/Model/nude mice分类
医药卫生引用本文复制引用
田树红,王日超,肖敏,符健..裸小鼠胃癌原位移植模型的建立[J].中国比较医学杂志,2017,27(2):21-24,4.基金项目
国家自然科学基金资助项目(NO81160408). （NO81160408）
