中国比较医学杂志2017，Vol.27Issue(2)：25-28,80,5.DOI:10.3969.j.issn.1671-7856.2017.02.005
斑马鱼几种病原菌监测
Monitoring of pathogenic bacteria in zebrafish
摘要
Abstract
Objective To conduct an epidemiological investigation of several pathogens in zebrafish, and to provide the basis for subsequent development of relevant standards. Methods This study mainly used traditional methods based on morphological, physiological and biochemical characteristics of bacteria, combined with molecular biology methods, to isolate bacteria and artificially infect healthy zebrafish with the bacteria. Through investigating the incidence and mortality in the experimental group, we distinguished the virulent effect of the pathogens, and further understanding the pathogens affecting the health of zebrafish. Results There were three kinds of pathogens identified in all sampling institutions, including pathogenic Aeromonas hydrophila, Aeromonas sobria and Pseudomonas aeruginosa. Aeromonas hydrophila was detected in most sampling institutions, and showed to possess strong virulence. Aeromonas sobria and Pseudomonas aeruginosa were detected in some sampling institutions, and the virulence of Aeromonas sobria was revealed to be weaker than that of Aeromonas hydrophila. The virulence of Pseudomonas aeruginosa was the weakest among these three bacteria, did not induce typical clinical symptoms and death in the zebrafish. Conclusions Aeromonas hydrophila may cause contamination of fish experimental facilities, and it has been shown to be potentially able to infect humans. Thus regarding the fish infection routes, enough attention should be payed to Aeromonas hydrophila, and it should be the focus in the pathogens monitoring.关键词
斑马鱼/病原体检测/PCR鉴定/人工感染Key words
Zebrafish/Pathogen detection/PCR identification/Artificial infection/分类
医药卫生引用本文复制引用
冯丽萍,杨迟,林金杏,胡建华..斑马鱼几种病原菌监测[J].中国比较医学杂志,2017,27(2):25-28,80,5.基金项目
国家科技支撑计划课题(No.2013BAK11B02)和上海市科技发展基金(No.15140901000)资助. （No.2013BAK11B02）
