中国比较医学杂志2017，Vol.27Issue(2)：44-48,5.DOI:10.3969.j.issn.1671-7856.2017.02.008
盐酸格拉司琼透皮贴剂在Beagle犬中多剂量给药的药代动力学研究
Pharmacokinetic study of granisetron hydrochloride transdermal patch by multiple administrations in beagle dogs
摘要
Abstract
Objective To establish an HPLC?MS/MS method for determination of the plasma concentration of granisetron hydrochloride administered by transdermal patch in Beagle dogs and evaluate its pharmacokinetics. Methods Six beagle dogs were given topically applied transdermal patches (72 h) for 6 times with an interval of 24 h. HPLC?MS/MS was applied to determine the concentration of granisetron hydrochloride in blood samples, thereafter the pharmacokinetic parameters were calculated. Results The trough concentration of Cmin was 0. 0459 ng/mL and 0. 2593 ng/mL after the first?dosage and sixth?dosage of granisetron transdermal patches, respectively. The peak concentration of Cmax was 0. 4637 ng/mL and 0. 4637 ng/mL, respectively. The AUC0~96 was 18. 92 h·ng/mL and 40. 21 h·ng/mL , respectively. The Tmax was 48. 3 hours and 45. 3 hours, respectively, and the MRT0-96 was 48. 9 hours and 45. 84 hours, respectively. Conclusions The concentration of granisetron has accumulation effect after repeated administration of granisetron hydrochloride transdermal patches, and it reaches a steady state on the sixteenth day by multiple administrations.关键词
盐酸格拉司琼透皮贴剂/beagle犬/HPLC⁃MS/MS/药代动力学Key words
Granisetron hydrochloride patch/Beagle dog/HPLC⁃MS/MS/Pharmacokinetics.分类
医药卫生引用本文复制引用
陈姗姗,孙莹莹,韦阳,王恪申..盐酸格拉司琼透皮贴剂在Beagle犬中多剂量给药的药代动力学研究[J].中国比较医学杂志,2017,27(2):44-48,5.基金项目
浙江省科技厅培育创新载体项目(编号:2015F10032) （编号:2015F10032）
浙江省中医药科学研究基金项目(编号:2011ZA017). （编号:2011ZA017）
