中国现代医学杂志2017，Vol.27Issue(3)：1-6,6.DOI:10.3969/j.issn.1005-8982.2017.03.001
莲子心提取物对博来霉素致小鼠肺纤维化的干预作用研究
Intervention of Plumula Nelumbinis extracts on Bleomycin-induced pulmonary fibrosis of mice
摘要
Abstract
Objective To investigate the effect of Plumula Nelumbinis extracts eluted with water (LEWW) or alcohol (LEAW) on Bleomycin-induced pulmonary fibrosis in mice.Methods The mouse model of pulmonary fibrosis was prepared by disposable intratracheal instillation of Bleomycin.The two kinds of Plumula Nelumbinis extracts were administrated orally for 21 days.After that,the lung tissues were obtained for HE staining and Masson staining;and inflammation score and the degree of pulmonary fibrosis,lung coefficient calculation,serum glutathione (GSH) and nitric oxide (NO) were evaluated.RT-PCR was used for detection of transforming growth factor-β1 (TGF-β1),smooth muscle actin (α-SMA),collagen-Ⅰ,collagen-Ⅲ mRNA expression levels.TGF-β1,α-SMA and hydroxyproline (HYP) protein expression levels in the lung tissues were measured using ELISA.Results Compared with the model group,the serum content of GSH increased,NO decreased;meanwhile the content of TGF-β1,α-SMA and HYP in the lung homogenate was lowered in the LEWW and LEAW groups (P ＜ 0.05).Conclusions Plumula Nelumbinis extracts have anti-fibrotic effect to Bleomycin-induced pulmonary fibrosis in mice.The mechanism may be related to the anti-oxidant activity of Plumula Nelumbinis extracts,inhibition of collagen formation and down-regulation of TGF-β1.关键词
莲心提取物/肺纤维化/胶原纤维/转化生长因子β1Key words
Plumula Nelumbinis extract/pulmonary fibrosis/collagen fiber/transforming growth factor-β1分类
医药卫生引用本文复制引用
李艳晓,高颖,王火,陈虹..莲子心提取物对博来霉素致小鼠肺纤维化的干预作用研究[J].中国现代医学杂志,2017,27(3):1-6,6.基金项目
国家自然科学基金(No:81503233) （No:81503233）
武警后勤学院院级课题(No:WHJ201510) （No:WHJ201510）
