中国现代医学杂志2017，Vol.27Issue(3)：7-12,6.DOI:10.3969/j.issn.1005-8982.2017.03.002
硫化氢通过p38MAPK和TGF-β1影响糖尿病小鼠心肌纤维化的研究
Hydrogen sulfide influences myocardial fibrosis in diabetic mice by p38MAPK and TGF-β1
摘要
Abstract
Objective To study the effect of exogenous hydrogen sulfide (H2S) on the expressions of p38 mitogen-activated protein kinase (p38MAPK) and transforming growth factor-beta1 (TGF-β1) and on the protection of diabetic myocardial fibrosis.Methods Eighteen male adult C57BL/6J mice were randomly divided into normal control group (NC group),diabetes mellitus group (ALX group),and diabetes mellitus treated with H2S group (ALX+H2S group) with six mice in each group.The diabetic mouse model was established by intraperitoneal injection (i.p.) of 200 mg/kg ALX.The mice in the normal control and ALX groups drank tap water freely everyday.The mice of the ALX+H2S group freely drank NaHS (H2S donor) solution at a concentration of 90 μmol/L.After 8 weeks,fasting blood glucose and serum lipid were tested.The pathological changes of myocardial fibers were observed by HE staining.The expressions of p-p38MAPK,TGF-β1 and collagen Ⅰ were detected using Western blot.The expressions of p38MAPK mRNA and TGF-β1 mRNA were tested by qRT-PCR.Results Compared with the NC group,the fasting blood glucose and serum lipid increased (P ＜ 0.05),the expression of collagen in myocardium increased and there was apparent myocardial interstitial fibrosis;the expressions of p-p38MAPK and TGF-β1 proteins and collagen Ⅰ increased (P＜ 0.05),the mRNA expressions of p38MAPK and TGF-β1 increased (P＜ 0.05) in the ALX group.After the intervention of H2S,the fasting blood glucose and serum lipid were improved (P＜ 0.05),the collagen fibers in the myocardial interstitium apparently decreased,the protein expressions of p-p38MAPK,TGF-β1 and collagen Ⅰ were decreased (P＜0.05),the mRNA expressions of p38MAPK and TGF-β1 were decreased (P ＜ 0.05).Conclusions H2S can ameliorate fasting blood glucose,serum lipid and myocardial fibrosis in diabetic mice,which might be related to the regulation of p38MAPK and TGF-β1 expressions.关键词
硫化氢/糖尿病心肌纤维化/p38丝裂原活化蛋白激酶/转化生长因子-β1/Ⅰ型胶原蛋白Key words
hydrogen sulfide/diabetic myocardial fibrosis/p38 mitogen-activated protein kinase/transforming growth factor-beta1/collagen Ⅰ分类
医药卫生引用本文复制引用
丁利,宋冰,张浩强..硫化氢通过p38MAPK和TGF-β1影响糖尿病小鼠心肌纤维化的研究[J].中国现代医学杂志,2017,27(3):7-12,6.基金项目
辽宁省自然科学基金(No:201602308) （No:201602308）
辽宁省博士启动课题(No:20131067) （No:20131067）
