中国现代医学杂志2017，Vol.27Issue(3)：13-17,5.DOI:10.3969/j.issn.1005-8982.2017.03.003
缺氧对细胞膜ABCA1降解的影响及其机制研究
Hypoxia enhances degradation of plasma membrane ABCA1 via increased calpain activity
摘要
Abstract
Objective To investigate the effect of hypoxia on the degradation of plasma membrane ATP binding cassette transporter A1 (ABCA1) and its calpain-related mechanism.Methods qRT-PCR was used to detect ABCA1 mRNA level in RAW264.7 cells stimulated by liver X receptor agonist TO-901317 for 24 h.Plasma membrane protein was extracted by the biotin labeling method of surface proteins,and membrane ABCA1 protein level was determined by Western blot.Western blot was applied to detect the plasma membrane ABCA1 protein level from TO-901317-treated RAW264.7 cells after 12-h hypoxia (1％ O2) treatment in the presence or absence of Cycloheximide.Furthermore,the calpain activity in the hypoxic cells was assayed by the method of Suc-LLVY-aminoluciferin.Finally,plasma membrane ABCA1 protein and calpain activity were measured in the RAW264.7 cells after intervention with calpain inhibitor ALLN.Results LXR agonist TO901317 up-regulated ABCA1 mRNA and membrane protein levels.Hypoxia reduced plasma membrane ABCA1 protein in the RAW264.7 cells and increased the calpain activity in the absence or presence of Cycloheximide.Calpain inhibitor ALLN,in part,reversed the decrease of plasma membrane ABCA1 induced by hypoxia.Conclusions Hypoxia might accelerate the degradation of plasma membrane ABCA1 via enhancement of calpain activity.关键词
三磷酸腺苷结合盒转运体A1/缺氧/钙蛋白酶/放线菌酮Key words
ATP binding cassette transporter A1/hypoxia/calpain/Cycloheximide分类
医药卫生引用本文复制引用
莫显刚,洪伟,王兰,张莉,刘大男,代陆军,蒋金,郜双林..缺氧对细胞膜ABCA1降解的影响及其机制研究[J].中国现代医学杂志,2017,27(3):13-17,5.基金项目
国家自然科学基金(No:31260250) （No:31260250）
