中国现代医学杂志2017，Vol.27Issue(3)：22-26,5.DOI:10.3969/j.issn.1005-8982.2017.03.005
外源性胰岛素样生长因子-1对老年小鼠认知功能的影响
Effect of exogenous insulin-like growth factors 1 on cognitive function in aged mice
摘要
Abstract
Objective To investigate the the effects of insulin-like growth factor 1 (IGF-1) on cognitive tunction in aged mice after microinjection of IGF-1.Methods IGF-1 was injected into the lateral ventricles of aged C57/BL6 mice.The expression of IGF-1R was assayed by Western Blot.The proliferative cells of the hippocampal dentate gyrus were marked by 5-bromodeoxyuridine (BrdU) immunofluorescence.The neurons of the hippocampal dentate gyms were determined by Nissl staining.Morris water maze and step-down test were performed to evaluate the cognitive function of the aged mice.Results Western blot results showed that the protein level of IGF-IR increased in the IGF-1 treated group compared with the control group (P＜0.05).BrdU immunofluorescent results revealed BrdU-positive cells were obviously increased in the IGF-1 treated group compared to the control group (P＜0.05).Nissl staining revealed the increased number of neurons in the IGF-1 treated group compared with the control group (P＜ 0.05).Morris water maze test showed that escape latency of the IGF-1 treated group was significantly shorter than that of the control group after training,and the number of times through the platform was significantly larger than that of the control group (P＜ 0.05).Step-down test showed that the number of times of basal errors was dramatically smaller,and the latency was significantly longer in the IGF-1 treated group compared with the control group (P＜ 0.05).Conclusions After injection of IGF-1,the neurocognitive functions of aged mice were improved,so we may indicate that IGF-1 may improve the cognitive function in aged mice.关键词
胰岛素样生长因子-1/老年鼠/5-溴脱氧尿嘧啶核苷/水迷宫/认知功能Key words
insulin-like growth factors 1/aged mice/5-bromo-2-deoxyuridine/Morris water maze/cognitive function分类
医药卫生引用本文复制引用
吴连连,袁红花,朱孝荣..外源性胰岛素样生长因子-1对老年小鼠认知功能的影响[J].中国现代医学杂志,2017,27(3):22-26,5.基金项目
江苏省自然科学基金(No:BK2010180、BK20151151) （No:BK2010180、BK20151151）
