中国现代医学杂志2017，Vol.27Issue(3)：27-33,7.DOI:10.3969/j.issn.1005-8982.2017.03.006
瑞舒伐他汀抑制血管平滑肌细胞表型转化的机制研究
Rosuvastatin inhibits phonotype transformation of vascular smooth muscle cells by down-regulation of KLF-4
韩庆 1沈德良 2王勃 2张金盈2
作者信息
- 1. 郑州大学附属郑州市中心医院 心血管内科,河南郑州450007
- 2. 郑州大学第一附属医院心血管内科,河南郑州450052
- 折叠
摘要
Abstract
Objective To verify whether Rosuvastatin could inhibit vascular smooth muscle cell (VSMC) phonotype switch by down-regulation of KLF-4.Methods The primary culture and identification of SD rat VSMCs were conducted,VSMCs in the 3rd to 5th passages were used for the following experiments.After treatment with different concentrations of Rosuvastatin,MTT was used to investigate the proliferative ability of VSMCs.Transwell chambers and wound healing were employed to test the migration ability of NSMCs.IHC was used to detect the expression of SM-actin and the morphological structure of VSMCs.Western blot was used to investigate the expressions of SM-actin,SM-MHC,SM-22α,osteopontin (OPN) and KLF-4 in VSMCs.After KLF-4 was transfected into VSMCs,the metheods were used again to test the phonotype changes of the VSMCs.Results Compared with the control group,the migration and proliferation ability of the VSMCs were decreased in the Rosuvastatin group.The expressions of SM-actin,SM-22α and SM-MHC in the Rosuvastatin group were significantly increased (P＜ 0.05),and the expression of OPN also increased (P＜ 0.05).IHC showed the VSMCs in the Rosuvastatin group became thinner and longer than those of the control group.After KLF-4 was transfected into VSMCs,the proliferation and migration ability of the VSMCs increased (P ＜ 0.05).Overexpression of KLF-4 reversed the effect of Rosuvsatatin on VSMCs phonotype transformation.Conclusions Rosuvastatin inhibits VSMCs phonotype switch by down-regulation of KLF-4.关键词
动脉粥样硬化/瑞舒伐他汀/血管平滑肌细胞/表型转化Key words
atherosclerosis/Rosuvastatin/VSMCs/phonotype switch分类
医药卫生引用本文复制引用
韩庆,沈德良,王勃,张金盈..瑞舒伐他汀抑制血管平滑肌细胞表型转化的机制研究[J].中国现代医学杂志,2017,27(3):27-33,7.
