中国现代医学杂志2017，Vol.27Issue(3)：34-40,7.DOI:10.3969/j.issn.1005-8982.2017.03.007
MicroRNA-222对肺腺癌细胞增殖、迁移和侵袭的影响
MicroRNA-222 promotes proliferation, migration and invasion of human lung adenocarcinoma cells by directly targeting ETS1
卢滨 1贾金广 1李利华 1姚菲菲1
作者信息
- 1. 河南省郑州人民医院呼吸内科,河南郑州450003
- 折叠
摘要
Abstract
Objective To investigate the role of microRNA-22 (miR-222) in the proliferation,migration and invasion of human lung adenocarcinoma cells.Methods Firstly,miR-222 and negative control were transfected into lung adenocarcinoma cells,and the CCK-8 assay,Transwell migration and Matrigel invasion assays were performed to detect the effect of miR-222 on cell proliferation,migration and invasion respectively.Then thc luciferase reporter assay,quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were performed to validate the putative target of miR-222.Subsequently,loss-of-function assay was applied to determine the target involved in the regulation of proliferation,migration and invasion of lung adenocarcinoma cells.Results The results of CCK-8 assay,Transwell migration and Matrigel invasion assays indicated that miR-222 could promote the proliferation,migration and invasion of lung adenocarcinoma cells.The luciferase reporter assay,qRT-PCR and Western blot showed that miR-222 directly targeted the 3'UTR of ETS1.And overexpression of ETS1 significantly inhibited the miR-222-induced proliferation,migration and invasion of lung adenocarcinoma ceils.Conclusions miR-222 facilitates proliferation,migration and invasion of lung adenocarcinoma cells by repressing ETS1.关键词
microRNA-222/ETS1/增殖/迁移/侵袭Key words
miR-222/ETS1/proliferation/migration/invasion分类
医药卫生引用本文复制引用
卢滨,贾金广,李利华,姚菲菲..MicroRNA-222对肺腺癌细胞增殖、迁移和侵袭的影响[J].中国现代医学杂志,2017,27(3):34-40,7.
