中国现代医学杂志2017，Vol.27Issue(3)：41-44,4.DOI:10.3969/j.issn.1005-8982.2017.03.008
超临界CO2萃取银杏叶中总黄酮醇苷的夹带剂工艺条件
Process condition of entrainer in extracting total favonol glycosides from Ginkgo leaves by supercritical CO2
刘雯 1李素娟 2马丹凤3
作者信息
- 1. 湖北省武汉市普爱医院药剂科,湖北武汉430032
- 2. 湖北省武汉市东西湖区人民医院药剂科,湖北武汉430040
- 3. 湖南省儿童医院药剂科,湖南长沙410007
- 折叠
摘要
Abstract
Objective To research the process condition of entrainer in extracting total favonol glycosides from Ginkgo leaves by supercritical CO2.Methods The optimum extraction technology condition was investigated by orthogonal experiment [L9(34)] with extraction rate of total favonol glycosides as the evaluation index,and with the addition way,kind,amount and flow rate of entrainer as investigation factors.The content of total favonol glycosides was determined by high performance liquid chromatography (HPLC).Results The adding way of entrainer is the presoak treatment plus dynamic extraction type,the entrainer was 95％ alcohol,with 300 ml infusing volume and 10 ml/min flowing rate.The best factors of supercritical fluid extraction were as follows:extraction pressure 20 MPa,extraction temperature 60℃,outlet temperature 70℃,collecting extracts with 95％ alcohol,dynamic extraction time 1 h.Under the processing conditions,the extraction rate of total favonol glycosides was 5.03％.Conclusions Supercritical CO2 extraction with the optimized process significantly improves the extraction of total flavonol glycosides from Ginkgo leaves.关键词
夹带剂/超临界CO2萃取/总黄酮醇苷/正交实验Key words
entrainer/supercritical CO2 extraction/total favonol glycosides/orthogonal experiment分类
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刘雯,李素娟,马丹凤..超临界CO2萃取银杏叶中总黄酮醇苷的夹带剂工艺条件[J].中国现代医学杂志,2017,27(3):41-44,4.
