中国现代医学杂志2017，Vol.27Issue(4)：1-6,6.DOI:10.3969/j.issn.1005-8982.2017.04.001
异氟醚对阿尔茨海默病APPswe/PS1dE9双转基因型小鼠学习记忆能力的影响
Influences of Isoflurane on learning and memory of APPswe/ PS1AE9 double transgenic mice of Alzheimer disease
摘要
Abstract
Objective To study the influences of Isoflurane on learning and memory of APPswe/PS1AE9 double transgenic mice of Alzheimer disease (AD). Methods Transgenic mice were divided into Tg-iso group and Tg-con group, wild-type mice were divided into Wt-iso group and Wt-con group. At the age of seven months, the Tg-iso group and the Wt-iso group were exposed to 1.1%Isoflurane for 2 hours per day for 5 days. Meanwhile, the mice in the Tg-con group and the Wt-con group inhaled oxygen/nitrogen (FiO2=30%). Learning and memory abilities were tested 48 hours and 5 months after Isoflurane exposure using the Morris Water Maze and Y maze, respectively. Results At the age of seven months, mean latency in the Wt-iso group decreased in a place navigation test compared to the Wt-con group ( P= 0.013); compared to the Tg-con group, the mean latency decreased in the Tg-iso group (P =0.043);there was no significant statistical difference in the target quadrant time or distance percentage in probe trials among the groups (P = 0.392). At the age of twelve months, compared to the Tg-con group, the discrimination errors of the Wt-con and Tg-iso groups decreased ( P= 0.044 and 0.020). Conclusions Repeated low-dose Isoflurane anaesthesia does not increase the dysfunction of learning and memory of APPswe/PS1AE9 double transgenic mice and wild type mice, to some extent, can even decrease dysfunction of learning and memory in APPswe/PS1AE9 double transgenic mice and wild type mice.关键词
学习记忆/异氟醚/APPswe/PS1dE9双转基因小鼠/Y迷宫/Morris水迷宫Key words
learning and memory/Isoflurane/APPswe/PS1AE9 double transgenic mice/Y-maze/Morris water maze分类
医药卫生引用本文复制引用
赵燕星,苏殿三,陈杰,王祥瑞..异氟醚对阿尔茨海默病APPswe/PS1dE9双转基因型小鼠学习记忆能力的影响[J].中国现代医学杂志,2017,27(4):1-6,6.基金项目
国家自然科学基金(No:30801069) （No:30801069）
上海市自然科学基金(No:08ZR1413700) （No:08ZR1413700）
教育部博士点基金(No:200802481094) （No:200802481094）
上海市科技启明星项目(No:09QA1403800) （No:09QA1403800）
