中国现代医学杂志2017，Vol.27Issue(4)：7-12,6.DOI:10.3969/j.issn.1005-8982.2017.04.002
脂肪干细胞与不同脱细胞基质的组织相容性研究
Histocompaticblity research of adipose stem cells with different acellular bladder matrix
摘要
Abstract
Objective To study the in vitro biocompatibility of SD rat adipose stem cells with different acellular bladder matrix (bladder acellular matrix graft, BAMG), and provide favorable experimental basis to further implantation into animals. Methods In the bilateral inguinal adipose tissue of SD rats, the primary fat cells were obtained after treatment. CD29, CD34, CD44 and CD45 were used to identify adipose derived stem cells by staining and flow cytometry. BAMG of SD rats and New Zealand rabbits were prepared. Using secondary precipitation method, adipose stem cells were inoculated on different BAMG surface, cell growth on the surface of the materials was observed, and cell growth curve was drawn. Results After in vitro culture of the cellular materials for 5 days, HE staining revealed the adipose derived stem cells of SD rats grew better on the surface of SD rat BAMG than on the surface of New Zealand rabbit BAMG, the cells grew into spindle cells, and the cell growth curve was consistent with that of the primary fat cells of SD rats. Conclusions Adipose stem cells of SD rats grow better on the surface of SD rat BAMG complex than on the surface of New Zealand rabbit BAMG complex, it has better biological compatibility. Our result provides favorable experimental basis for further implantation of the cell scaffold complex into animals.关键词
脂肪干细胞/脱细胞膀胱基质/组织相容性/修复Key words
adipose stem cell/bladder acellular matrix graft/tissue compatibility/repair分类
医药卫生引用本文复制引用
白勇,武阳,凯赛尔·阿吉,乔炳璋,王玉杰,木拉提·热夏提..脂肪干细胞与不同脱细胞基质的组织相容性研究[J].中国现代医学杂志,2017,27(4):7-12,6.基金项目
国家自然科学基金(No:81160088) （No:81160088）
