中国现代医学杂志2017，Vol.27Issue(4)：13-16,4.DOI:10.3969/j.issn.1005-8982.2017.04.003
不同品系小鼠肺纤维化模型的比较研究
Comparative study on pulmonary fibrosis model induced by Bleomycin in three strains of mice
摘要
Abstract
Objective To find out the most proper model of human pulmonary fibrosis through a comparative study of pulmonary fibrosis in three strains of mice induced by Bleomycin (BLM). Methods C57BL/6, BALB/c and KM mice were randomly divided into model groups and normal control groups, respectively. Three model groups received a single intratracheal instillation of 5 mg/kg body weight of BLM to induce pulmonary fibrosis, while normal control groups were administrated the same amount of normal saline. On the 7th, 14th and 28th day, 8 mice were respectively sacrificed in each model group. Lung tissues were removed to measure the lung coefficient and hydroxyproline content of the mice at different time points, and Masson staining was used to evaluate the extent of pulmonary fibrosis. Results The lung coefficient, hydroxyproline content and pulmonary histopathological changes of each model group were remarkably different from those of the corresponding normal control group (P < 0.05). Compared with the BALB/c and KM model groups, the lung coefficient, hydroxyproline content and pulmonary histopathological changes of the C57BL/6 model group were significantly increased (P <0.05). Conclusions C57BL/6 is more suitable for the model of pulmonary fibrosis in the three strains of mice because of sensitive Bleomycin reaction and obvious pulmonary fibrosis. Of the three strains, C57BL/6 is the most suitable model in studying pulmonary fibrosis.关键词
肺纤维化/博来霉素/小鼠Key words
pulmonary fibrosis/Bleomycin/mouse分类
医药卫生引用本文复制引用
宋桂芹,徐志伟,李泽,李向上,张效云..不同品系小鼠肺纤维化模型的比较研究[J].中国现代医学杂志,2017,27(4):13-16,4.基金项目
张家口市科技局自然科学基金资助项目(No:-12110046D-1) （No:-12110046D-1）
