中国现代医学杂志2017，Vol.27Issue(4)：22-26,5.DOI:10.3969/j.issn.1005-8982.2017.04.005
组织型纤溶酶原激活物突变体对鼠急性脑梗死及脑组织保护作用实验研究
Protective effect of tissue type plasminogen activator mutant on acute cerebral thrombosis and cerebral tissue in rats
侯沛秋 1祝丹 1唐曦 2林川 3曾大奎3
作者信息
- 1. 成都医学院 医学影像系,四川 成都 610500
- 2. 成都医学院第一附属医院 医学影像系,四川 成都 610500
- 3. 四川省宜宾市第二人民医院 肿瘤二科,四川 宜宾 644000
- 折叠
摘要
Abstract
Objective To evaluate the thrombolytic and brain-protective effect of new tissue plasminogen activator mutant in rat model of acute cerebral thrombosis. Methods Eighty-four adult Wistar rats were randomly divided into control group, tissue-type plasminogen activator (t-PA) group, low-dose tissue plasminogen activator mutant (low-dose t-PAm) group and conventional-dose tissue plasminogen activator mutant (regular-dose t-PAm) group. The rats of the three groups were treated separately 3 hours after thrombosis in middle cerebral artery. Volume of infarction, neurologic scores and severity of hemorrhage were observed 24 hours after treatment. The protective role of t-PAm for brain tissue was evaluated through neutrophil infiltration and the change in the concentration of PAR-1. Results Volume of infarction in the low-dose t-PAm group and the regular-dose t-PAm group was significantly smaller than that in the control group [(108.5 ± 27.3) mm3 and (68.3 ± 17.2) mm3 vs (323.4 ± 42.3) mm3], and the neurologic scores were evidently correlated with the volume of infarction ( r= 0.613,P =0.000), while the incidence of cerebral hemorrhage in the low-dose t-PAm group was not significantly increased. T-PAm also reduced the production of myeloperoxidase, as well as the production of PAR-1, compared with the t-PA group (P <0.001). Conclusions New tissue plasminogen activator mutant shows better thrombolytic effect with significant protection for ischemic brain tissue.关键词
尿激酶/血栓/组织型纤溶酶原激活物/突变体Key words
urokinase/thrombus/recombinant tissue-type plasminogen activator/mutant分类
医药卫生引用本文复制引用
侯沛秋,祝丹,唐曦,林川,曾大奎..组织型纤溶酶原激活物突变体对鼠急性脑梗死及脑组织保护作用实验研究[J].中国现代医学杂志,2017,27(4):22-26,5.
