中国现代医学杂志2017，Vol.27Issue(4)：27-32,6.DOI:10.3969/j.issn.1005-8982.2017.04.006
MicroRNA-150-5p对肝细胞癌细胞增殖、凋亡和周期的影响
Effects of miR-150-5p on proliferation, apoptosis and cell cycle of hepatocellular carcinoma cells
赵月秋 1张正宝 1郭红霞 1詹传飞 2鲁皓 2钱晓峰2
作者信息
- 1. 江苏联合职业技术学院南京卫生分院,江苏 南京 210038
- 2. 江苏省人民医院 肝脏外科,江苏 南京 210029
- 折叠
摘要
Abstract
Objective To investigate the expression and effect of miR-150-5p on cell proliferation, cell apoptosis and cell cycle in human hepatocellular carcinoma cells. Methods The expression of miR-150-5p in hepatocellular carcinoma cells and normal human liver cells was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Proliferation of hepatocellular carcinoma cells was analyzed by MTT. Cell apoptosis and cell cycle of hepatocellular carcinoma cells were analyzed b y flow cytometry. Luciferase assay was performed to detect whether ZNF609 was the direct target of miR-150-5p in the hepatocellular carcinoma cells. Results The expression of miR-150-5p was significantly down regulated in the hepatocellular carcinoma cells compared to the normal human liver cells. Proliferation of the hepatocellular carcinoma cells was significantly inhibited by miR-150-5p, but cell apoptosis was significantly promoted. Cell cycle of the hepat ocellular carcinoma cells was arrested at G1 phage after transfection of miR-150-5p mimics. Further investigation proved ZNF609 was the direct target of miR-150-5p in hepatocellular carcinoma cells. Conclusions miR-150-5p may function as tumor suppressor and play important roles in cell proliferation, cell apoptosis and cell cycle in hepatocellular carcinoma cells by directly targeting ZNF609.关键词
MicroRNA-150-5p/增殖/凋亡/周期/ZNF609Key words
miR-150-5p/cell proliferation/cell apoptosis/cell cycle/ZNF609分类
医药卫生引用本文复制引用
赵月秋,张正宝,郭红霞,詹传飞,鲁皓,钱晓峰..MicroRNA-150-5p对肝细胞癌细胞增殖、凋亡和周期的影响[J].中国现代医学杂志,2017,27(4):27-32,6.
