中国现代医学杂志2017，Vol.27Issue(5)：7-12,6.DOI:10.3969/j.issn.1005-8982.2017.05.002
葛根素通过p38MAPK通路抑制MC3T3-E1成骨细胞凋亡
Puerarin inhibited MC3T3-E1 human osteoblast clees apoptosis through P38MAPK pathway
摘要
Abstract
Objective To detect the mechanism of Puerarin inhibited Dexamethasone-induced MC3TE-E1 cells apoptosis . Methods The MC3TE- E1 cells were cultured and passaged , and MTS and immune fluo rescence were used to detect proliferation effect of different concentration of Puerarin and Dexamethasone in MC3TE-E1 cells and the the protect effect of Puerarin in MC3TE-E1 cells respectively. Western blot was used to detect the p38MAPK, p-p38MAPK protein expression. Realtime PCR were used to detect Caspase-3 gene expression. ELISA detection was used to clarified the anti-apoptotic effect of p38MAPK inhibitior, SB203580, on cells apoptosis. Results MTS results showed that 10-7, 10-8 mol/L puerarin increased the cell proliferation significantly, and the concentration of DEX at 10-6, 10-7 mol/L after treatment of 48 h inhibited the cell proliferation. Immunofluorescence results showed that 10-8 mol/L puerarin could inhibited DEX-induced cells apoptosis. Western blot results showed after DEX treatment, the expression of p-p38MAPK increased, and after DEX and PUE cotreatment, DEX induced p-p38MAPK phosphorylation was inhibited. Realtime PCRshowed gene expression of Caspase-3 mRNA was up-regulated after DEX treatment, while gene expression of Caspase-3 mRNA down-regulated after cultured with DEX and PUE. ELISA showed that cells apoptosis in-creased after DEX treatment alone, when combined wit PUE, cells apoptosis was reduced to the normal levels, and when combined SB203580 with DEX, cells apoptosis induced by DEX were abolished partially. Conclusions Puerarin inhibits dexamethasone-induced MC3TE-E1 cells apoptosis through p38MAPK pathway.关键词
葛根素/地塞米松/凋亡/p38MAPKKey words
Puerarin/Dexamethasone/Apoptosis/P38MAPK分类
医药卫生引用本文复制引用
于冬冬,赵丹阳..葛根素通过p38MAPK通路抑制MC3T3-E1成骨细胞凋亡[J].中国现代医学杂志,2017,27(5):7-12,6.基金项目
辽宁省教育厅(No:L201621) （No:L201621）
