中国现代医学杂志2017，Vol.27Issue(5)：13-17,5.DOI:10.3969/j.issn.1005-8982.2017.05.003
负载牙周祖细胞的钛种植体治疗单根牙缺失的实验研究
Effect of bioengineered periodontal tissue on titanium dental implant
刘艳玲 1牟雁东1
作者信息
- 1. 西南医科大学 口腔医学院,四川 泸州 646000
- 折叠
摘要
Abstract
Objective To investigate the ability of autologous dental progenitor formed bioengineered perio-dontal tissue on titanium dental implants. Methods The Lewis rats maxillary first and second molars (M1 and M2) were extracted and the periodontal ligament (PDL)-derived dental progenitor cells (DPCs) were harvested and cultured. The proliferation, alkaline phosphatase activity, colony - forming and differentiation potential of DPCs were detected. Implants were prepared by DPC, and placed at the healed M1 extraction site of each rat host. Bioengineered PDL tissues were evaluated morphologically and histomorphometricly. Results Cultured PDL-derived DPCs exhibited high proliferation rates, clonogenicity, and adipose, bone, and neural cell differ-entiation. Bioengineered periodontal tissues formed cementum-like tissue on the titanium implant surface. PDL tissue with sharpey's fibers inserted perpendicular to the implant. Conculsions PDL-derived DPCs could potentially be used to regenerate autologous PDL tissues on titanium implants grown in the jaw.关键词
牙周膜/生物工程组织/钛种植体Key words
periodontal ligament/bioengineered tissue/titanium dental implant分类
医药卫生引用本文复制引用
刘艳玲,牟雁东..负载牙周祖细胞的钛种植体治疗单根牙缺失的实验研究[J].中国现代医学杂志,2017,27(5):13-17,5.
