中国现代医学杂志2017，Vol.27Issue(5)：18-23,6.DOI:10.3969/j.issn.1005-8982.2017.05.004
被转化生长因子β活化的长链非编码RNA对人淋巴瘤Raji细胞增殖、周期及凋亡的影响
Effect of LncRNA-ATB on proliferation, apoptosis and cell cycle of Raji cell
赵伟 1李晓明2
作者信息
- 1. 西南医科大学,四川 泸州 646000
- 2. 西南医科大学附属医院 血液内科,四川 泸州 646000
- 折叠
摘要
Abstract
Objective To investigate effects of long non-coding RNA activated by TGF-β (LncRNA-ATB) on cell proliferation, apoptosis, and cell cycle of human lymphoma Raji cell. Methods The efficiency of LncRNA-ATB shRNA, miR-200c mimics, and the expressions of LncRNA-ATB, miR-200c, and KRAS were detected by quantitative real time PCR (qRT-PCR). The cell proliferation of Raji transfected with LncRNA-ATB shRNA and NC were analyzed by MTT assays, and the cell apoptosis and cell cycle of Raji cells transfected with LncRNA-ATB shRNA and NC were analyzed by flow cytometry. Luciferase assays were per-formed to detect whether KRAS was the direct target of miR-200c. Results The expression of LncRNA-ATB could be effectively silenced by LncRNA-ATB-shRNA. Compared with Raji cells transfected with LncRNA-ATB-NC, cell proliferation significantly inhibited in Raji cells transfected with LncRNA-ATB-shRNA, but cells apoptosis was significantly promoted. Cell cycle was arrested at G1 phage in Raji cells transfected with LncRNA-ATB-shRNA. Further investigation found that LncRNA-ATB could promote the proliferation of Raji cells and function as ceRNA to upregulate the expression of KRAS by competed with miR-200c. Conclusions LncRNA-ATB may play an important role in the proliferation, apoptosis and cell cycle of Raji cells by upreg-ulating the expression of KRAS.关键词
被转化生长因子β活化的长链非编码RNA/Raji细胞/鼠类肉瘤病毒癌基因/miR-200c/增殖Key words
LncRNA-ATB/Raji cell/kirsten rat sarcoma viral oncogene/miR-200c/proliferation分类
医药卫生引用本文复制引用
赵伟,李晓明..被转化生长因子β活化的长链非编码RNA对人淋巴瘤Raji细胞增殖、周期及凋亡的影响[J].中国现代医学杂志,2017,27(5):18-23,6.
