中国现代医学杂志2017，Vol.27Issue(6)：1-5,5.DOI:10.3969/j.issn.1005-8982.2017.06.001
环磷酸腺苷信号对矽肺纤维化形成的影响
Influence of cAMP signal on pathogenesis of silicotic fibrosis
摘要
Abstract
Objective To observe the changes of cyclic adenosine monophosphate (cAMP) signal in patho-genesis and development of silicotic fibrosis. Methods A HOPE-MED8050 exposure control apparatus was used to copy the silicosis model. Male Wistar rats were randomly divided into 6 groups (10 in each group) and the rats inhaled dust respectively for 0, 2, 4, 8, 12 and 16 w. Masson trichrome staining was performed to observe the histomorphology of the lung tissues. The expressions of α smooth muscle actin (α-SMA), col-lagen Ⅰ, fibronectin (Fn), stimulatory G protein α (Gαs), inhibitory G protein α (Gαi2/3) and cAMP were detected by immunohistochemistry and Western blot respectively. Results Masson trichrome staining revealed bluish-purple collagen deposition in the 4-w silicosis group, and the collagen deposition in the fibrotic lesions increased with the time of exposure to silica. Immunohistochemical results showed that in the control group positive α-SMA expression was seen in the trachea and vascular smooth muscles, while in the 16-w silicosis group α-SMA was observed around the fibrotic nodules and in the interstitial fibrotic area. The expressions ofα-SMA, collagen I, Fn and Gαi2/3 proteins gradually increased in the 4-w, 8-w, 12-w and 16-w silicosis groups compared with the control group. As compared to the control group, the expressions of Gαs and cAMP significantly decreased in the 8-w silicosis group, and were the lowest in the the 16-w silicosis group. Conclusions cAMP expression decreases along with the pathogenesis and development of silicosis, and cAMP has a significant role in silicotic formation.关键词
矽肺病/肌成纤维细胞/环磷酸腺苷Key words
silicosis/myofibroblast/cyclic adenosine monophosphate分类
医药卫生引用本文复制引用
丁咏伟,耿玉聪,刘燕,徐洪,张丽娟,王瑞雪,王建辉,胡启峰,杨方..环磷酸腺苷信号对矽肺纤维化形成的影响[J].中国现代医学杂志,2017,27(6):1-5,5.基金项目
国家自然科学基金(No:81472953) （No:81472953）
河北省自然科学基金青年基金(No:H2016209161) （No:H2016209161）
华北理工大学大学生创新计划(No:X2016218) （No:X2016218）
