中国现代医学杂志2017，Vol.27Issue(6)：6-9,4.DOI:10.3969/j.issn.1005-8982.2017.06.002
慢病毒转染和电转染原代C57BL/6小鼠神经干细胞的比较
Comparison of lentivirus transfection and electroporation transfection in primary neural stem cells of C57BL/6 mice
摘要
Abstract
Objective To develop an efficient method to transfect mouse primary neural stem cells (NSCs). Methods Primary neural stem cells were isolated from brain tissue of C57BL/6 mice, then, lentivirus transfection and electroporation methods were used to transfect primary NSCs. By observing the green fluorescent protein expression the transfection effect of the two methods was compared. The survival rate of primary NSCs was assessed using MTT method, the effect of the two methods on primary NSCs were compared. Results The rate of lentivirus transfection [(77.6 ± 6.6)%] was higher than that of electroporation [(29.2 ± 4.8)%, P<0.05] 48 h after transfection. After 72 h, the amount of green fluorescence expression was (60.0 ± 3.6)% by lentivirus transfection and only (12.8 ± 2.4)% by electroporation method. The survival rate of lentivirus transfection [(70.1 ± 1.4)%] was not significantly lower than that of electroporation [(75.8 ± 2.3)%, P>0.05]. Conclusions Lentivirus transfection is more efficient than electroporation for NSCs.关键词
神经干细胞/慢病毒转染/电转染Key words
neural stem cell/lentivirus transfection/electroporation分类
医药卫生引用本文复制引用
加三三,胡俊,李美宁,张凯丽,刘志贞..慢病毒转染和电转染原代C57BL/6小鼠神经干细胞的比较[J].中国现代医学杂志,2017,27(6):6-9,4.基金项目
国家自然科学基金(No:81300487) （No:81300487）
山西省回国留学人员科研资助项目(No:2016-51) （No:2016-51）
