中国药科大学学报2017，Vol.48Issue(1)：76-81,6.DOI:10.11665/j.issn.1000-5048.20170112
百部生物碱对博来霉素诱导肺纤维化小鼠的保护作用
Protective effects of stemona alkaloids on mice with bleomycin-induced pul-monary fibrosis
摘要
Abstract
This study aimed to investigate the protective effect of total alkaloids ( TA) and one of the active com-ponents, neotuberostemonine, of Stemona tuberosa on bleomycin-induced pulmonary fibrosis in mice and to explore the mechanism by fibroblasts model. The bleomycin-induced mice were orally administered with TA ( 60 mg/kg) and neotuberostemonine ( 10, 20 mg/kg) , with prednisone ( 6. 67 mg/kg) as a positive control. The anti-fibrotic effects were assessed by hydroxyproline content, TGF-β1 level, inflammatory score, collagen deposition and the expression of α-SMA in the lung tissues. The results revealed that TA and neotuberostemonine could significantly ameliorate the inflammation and injury, and attenuate the hydroxyproline content and collagen deposition. Moreover, neotuberostemonine treatment markedly down-regulated the α-SMA level and TGF-β1 content in bleomycin-injured mice lungs. The in vitro experiments showed that neotuberostemonine inhibited the expression ofα-SMA induced by TGF-β1 in a dose-dependent manner, indicating that suppression on differentiation of fibroblasts to myofibroblasts may be one of the mechanisms for neotuberostemonine against pulmonary fibrosis.关键词
对叶百部/总生物碱/新对叶百部碱/肺纤维化/肺成纤维细胞Key words
Stemona tuberosa/total alkaloids/neotuberostemonine/pulmonary fibrosis/pulmonary fibroblasts分类
医药卫生引用本文复制引用
向娟,余平,李明丹,张朝凤,许翔鸿,张勉..百部生物碱对博来霉素诱导肺纤维化小鼠的保护作用[J].中国药科大学学报,2017,48(1):76-81,6.基金项目
国家“重大新药创制”科技重大专项资助项目(No．2011ZX09307-002-02)This work was funded by the National Science and Technology Major Project for New Drugs Development ( No．2011ZX09307-002-02) （No．2011ZX09307-002-02）
