中国烟草科学2017，Vol.38Issue(1)：1-7,7.DOI:10.13496/j.issn.1007-5119.2017.01.001
普通烟草DREB转录因子的序列鉴定与表达分析
Genome-wide Sequence Identification and Expression Analysis of DREB Transcription Factors in Nicotiana tabacum
摘要
Abstract
DREB transcription factors,with a typical AP2/ERF DNA-binding domain,play a key role in plants' tolerance to environmental stresses.In this study,based on the common tobacco genome database,68 members of the tobacco DREB gene family were identified according to Arabidopsis thaliana protein sequences.The analyses of physical and chemical properties,phylogenetic tree,gene structures,and tissue expression patterns were performed.The results suggested that the peptide length of different subfamily members was significantly different.Phylogenetic analysis revealed that the tobacco DREB transcription factor family members could be divided into 5 subfamilies,lacking the A6 group from Arabidopsis thaliana.The patterns of protein structures and organization of conserved domains in tobacco were highly conserved within subfamilies based on the phylogenetic results.Transcriptome analysis showed that the expression pattems of DREBs were different in different tissue types and most DREBs have low expression levels in tobacco,although some have higher expression levels after environmental stress treatments.These results provide a very useful reference for cloning and functional analysis of DREB genes in tobacco.关键词
DREB转录因子/普通烟草/生物信息学/表达分析Key words
DREB transcription factor/Nicotiana tabacum/bioinformatics/expression analysis分类
农业科技引用本文复制引用
胡军华,王大伟,杨明磊,王新,吴新儒,吴华,刘贯山..普通烟草DREB转录因子的序列鉴定与表达分析[J].中国烟草科学,2017,38(1):1-7,7.基金项目
国家烟草专卖局烟草基因组计划重大专项"烟草高香气、抗青枯病、抗TMV突变体鉴定及中烟100、翠碧一号品种定向改良" [110201301005(JY-05)] （JY-05）
