中国烟草学报2017，Vol.23Issue(1)：8-14,7.DOI:10.16472/j.chinatobacco.2016.291
胶体金免疫层析法快速检测烟叶中三唑酮残留量
Rapid detection of triadimefon residues in tobacco leaves with colloidal gold immunochromatography
摘要
Abstract
Gold immunochromatography assay was adopted to establish a rapid detection method for triadimefon residues in tobacco leaves.Colloidal gold was prepared by sodium citrate method,and was then conjugated to triadimefon antibodies.Triadimefon-OVA and anti-mouse goat immunoglobulins were jet-positioned onto a nitrocellulose membrane.Sample pad,gold jed pad,nitrocellulose membrane and absorbent paper were assembled and cut into detecting card,respectively.Results showed that the sensitivity of rapid triadimefon visual detection limit is 1 mg/kg for residues of triadimefon and triadimenol in tobacco leaves,and detection time was 5～10 min.Repeatability was good,and it gave almost no false positive or negative results compared with gas chromatography-tandem mass spectrometry.关键词
胶体金免疫层析法/烟叶/农药残留/三唑酮Key words
gold immunochromatography/tobacco leaf/pesticide residue/triadimefon引用本文复制引用
楼小华,高川川,朱文静,巴金莎,扶胜,杨梅,易重任..胶体金免疫层析法快速检测烟叶中三唑酮残留量[J].中国烟草学报,2017,23(1):8-14,7.基金项目
贵州省烟草专卖局(公司)科技项目(No.201518) （公司）
