中国烟草学报2017，Vol.23Issue(1)：27-34,8.DOI:10.16472/j.chinatobacco.2016.086
免试剂离子色谱(RFIC)法测定卷烟主流烟气总粒相物中的烟碱
Determination of nicotine in total particulate matter of mainstream cigarette smoke by reagent-free ion chromatography
摘要
Abstract
A method was developed for separation and determination of nicotine by reagent free ion chromatography (RFIC) coupled to ultraviolet detection with external calibration.Separation was achieved by gradient elution with a carboxylate-functionalized cationexchange column CS 19 in a short time.Under the optimum elution conditions,no interferences existed between nicotine and anatabine (or nomicotine).The relative standard deviations for nicotine standard solution and cigarette sample were 0.49％ and 2.3％ (n=5),respectively.The linear range of nicotine was 1.00 ～ 500.0 μg/mL,with correlation coefficient (R) of 0.9999.The limit of detection (LOD) in terms of signal-to-noise of 3∶1 is 0.8 μg/cig.The spiked recovery ranged from 93.2％ to 101.7％.Two certified reference cigarettes (CRCs) from China Tobacco Standardization Research Center were determined.The determined results and certified values were 0.588,0.942 mg/cig and 0.60±0.085,1.00±0.137 mg/cig,respectively,indicating good accuracy of the method.The present method was proved a potential alternative to gas chromatography (GC),high performance liquid chromatography (HPLC),and ultra performance liquid chromatography (UPLC) method.关键词
烟碱/免试剂离子色谱法/测定/总粒相物Key words
nicotine/reagent free ion chromatography/determination/total particulate matter引用本文复制引用
李力,李东亮,张威,胡清源,田永红..免试剂离子色谱(RFIC)法测定卷烟主流烟气总粒相物中的烟碱[J].中国烟草学报,2017,23(1):27-34,8.基金项目
中国烟草总公司减害技术重大专项项目“降低烟气中氨含量的材料筛选、合成及应用”[110201101027(JH-02)] （JH-02）
