中国烟草学报2017，Vol.23Issue(1)：86-94,9.DOI:10.16472/j.chinatobacco.2016.228
β-氨基丁酸诱导烟草抗碱初步研究
Preliminary study on BABA-induced resistance to alkaline stress in tobacco
摘要
Abstract
This study introduced the function of BABA in regulating tobacco on alkaline stress and attempted to explore the mechanism through antioxidant system,iontransport and related genes expression.Results showed that both 0.2mmol/L and 0.5mmol/L BABA could significantly increase root length and fresh weight of tobacco under alkaline stress.BABA could increase contents of GSH,ASA,chlorophyll,carotenoid and activities of SOD,APX,POD and CAT while decrease contents of H2O2,proline,MDA and relatively conductivity rate.Decreasing Na+ content and increasing K+ content were to defense ion toxicity and increasing organic acid content could defend high pH toxicity under BABA treatment.Result of real-time relative quantitative PCR showed that BABA could induce NtCaM and ABC translocator genes up-regulated expression to regulate transport and signal transduction and it also induced GSH1and PROX1 genes up-regulated expression to promote the GSH synthesis and proline metabolism.The above evidences suggested that BABA-induced resistance to alkaline stress in tobacco resulted from integrated effect of antioxidant,organic acid synthesis,ion transport and gene expression.关键词
β-氨基丁酸/烟草/碱胁迫Key words
β-aminobutyric acid/tobacco/alkaline stress引用本文复制引用
尤本武,董建江,刘紫薇,杨波,郭家明,陈学平..β-氨基丁酸诱导烟草抗碱初步研究[J].中国烟草学报,2017,23(1):86-94,9.基金项目
江西省烟草公司“提高烟草抗逆性的新型调节物质研制及应用”(赣2011.01.001号)和“基于氯化血红素的高效生长调节剂的研制”(皖0920140421003号) （赣2011.01.001号）
