中国烟草学报2017，Vol.23Issue(1)：95-101,7.DOI:10.16472/j.chinatobacco.2016.263
具翼烟草(Nicotiana alata)核型、C带与rDNA染色体定位研究
Study on karyotype, C-banding and rDNA chromosome locus in Nicotiana alata
摘要
Abstract
The karyotype,C-banding,and chromosome locus of 45S and 5S rDNA of Nicotiana alata were studied by methods of wall digestion and hypotonic treatment,BSG,and double color fluorescent in situ hybridization,respectively.Karyotype analysis of root tip cells of four N.alata accessions showed that the average karyotype formula was 2n =2x =12m + 6st,the variation and average value of arm ratio was 1.14～4.22 and 2.17,respectively.The rate of the longest and minimum chromosome was 2.16,and the karyotype classification was 2B of Stebbins system (1971).Results of C-banding study showed that chromosome C-banding of N.alata was abundant with 25 bands being displayed,including 7 centromeric bands,15 terminal bands,and 3 intercalary bands.Results of FISH study showed,in metaphase chromosomes of root tip cell ofN.alata,there were three pairs of 45S rDNA loci,locating on ⅠS,ⅢS,and ⅤL,respectively (Land S represented chromosome long and short arm respectively,and the Roman number indicated chromosomes serial number;the same hereinafter),and two pairs of 5S rDNA loci,locating on ⅠL and ⅣS,respectively.Pair Ⅰ of homologous chromosome had loci of both 45S and 5S rDNA.These results could provide reference for studies on phylogenesis of genus Nicotiana,and were of great significance to genetic breeding and germplasm innovation in tobacco.关键词
烟草/核型/染色体/荧光原位杂交/C带Key words
Nicotiana/karyotype/chromosome/FISH/C-banding引用本文复制引用
王霖娇,任学良,盛茂银,杜家颖,温培才..具翼烟草(Nicotiana alata)核型、C带与rDNA染色体定位研究[J].中国烟草学报,2017,23(1):95-101,7.基金项目
烟草行业重点实验室专项经费资助项目(中烟办[2014]5号),国家重点研发计划项目课题(2016YFC0502603),贵州省社会发展攻关计划项目(黔科合SZ字[201413036号) （中烟办[2014]5号）
