中国药房2017，Vol.28Issue(7)：881-884,4.DOI:10.6039/j.issn.1001-0408.2017.07.05
银杏叶聚戊烯醇对Aβ25-35诱导dPC12细胞损伤的保护作用研究
Study on Protective Effects of the Polypentenol from Ginkgo biloba Leaf on Aβ 25-35-induced Damage in dPC12 Cells
摘要
Abstract
OBJECTIVE:Study the protective effect of the polypentenol from Ginkgo biloba leaf(GP)on Aβ25-35-induced dam-age in dPC12 cells ,and to provide reference for the treatment of Alzheimer's disease(AD). METHODS:After PC12 cells were dif-ferentiated into dPC12 cells with neural activity by incubating with never growth factor(NGF),the dPC12 cells were divided into normal control group(DMSO medium),Aβ25-35 treatment group(DMSO medium)and GP test group(DMSO medium respectively containing 25,50,100,200,400 μg/mL GP). After 24 h cultivating,cells in Aβ25-35 treatment group and GP test group were added 25 μmol/L Aβ25-35 to induce cell damage(AD cell model),MTT method was used to determine the survival rate after 24 h. Other cells were divided into normal control group(DMSO medium),Aβ25-35 treatment group(DMSO medium)and GP test group(DMSO medium respectively containing 25,50,100,200 μg/mL GP),the lactate dehydrogenase(LDH),reactive oxygen species(ROS), malondialdehyde (MDA) levels in cell culture medium were detected after the same treatment. RESULTS:Compared with Aβ25-35 treatment group,the survival rates of dPC12 cells in 50,100,200,400 μg/mL GP test groups were obviously increased(P<0.05 or P<0.01);LDH,ROS,MDA levels in 100,200 μg/mL GP test groups and ROS level in 50 μg/mL GP test group were signifi-cantly reduced(P<0.05 or P<0.01),showing a certain concentration-dependence. CONCLUSIONS:GP has certain protective ef-fect on Aβ25-35-induced damage in dPC12 cells,and it's a potential drug for AD.关键词
银杏叶/聚戊烯醇/β淀粉样肽25-35/dPC12细胞/乳酸脱氢酶/活性氧/丙二醛Key words
Ginkgo biloba leaf/Polypentenol/Aβ25-35/dPC12 cell/Lactate dehydrogenase/Reactive oxygen species/Malondi-aldehyde分类
医药卫生引用本文复制引用
陈卫卫,梁迪,赵其秀,罗毅,刘灵杰,莫丽萍,黄俊善,李海涛..银杏叶聚戊烯醇对Aβ25-35诱导dPC12细胞损伤的保护作用研究[J].中国药房,2017,28(7):881-884,4.基金项目
广西自然科学基金面上项目(No.2013GXNSFAA-019115) （No.2013GXNSFAA-019115）
广西壮族自治区卫生厅中医药科技专项课题(No.GZZY13-09) （No.GZZY13-09）
壮瑶药协同创新中心课题(No.桂教科研﹝2013﹞20号) （No.桂教科研﹝2013﹞20号）
广西壮瑶药重点实验室课题(No.桂科基字﹝2014﹞32号) （No.桂科基字﹝2014﹞32号）
