中国药房2017，Vol.28Issue(7)：957-960,4.DOI:10.6039/j.issn.1001-0408.2017.07.27
三黄益肾方的纯化工艺研究
Study on the Purification Technology of Sanhuang Yishen Formula
摘要
Abstract
OBJECTIVE:To study the purification technology of Sanhuang yishen formula. METHODS:Using retention rate and impurity rate of purified total polysaccharide,astragaloside and calycosin glucoside as index,the purification effects of water extraction and alcohol precipitation method (50%,60%,70% ethanol) and clarifying agent method (101 juice clarifying agent, ZTC natural clarifying agent,chitosan clarifying agent) were respectively detected to screen the purification method;orthogonal test was used to optimize the technology parameters(mass concentration of liquid,amount of clarifying agent and pH of liquid)by the optimized purification method,and the verification test was conducted. RESULTS:The purification was better when using chito-san as clarifying agent with comprehensive score of 98.62;the purified technology parameters were mass concentration of liquid 1 g/mL,1% chitosan solution amount of 2 mL/g,pH 5.1;the average value of retention rate and impurity rate of purified total poly-saccharide, astragaloside and calycosin glucoside in verification test were 79.56%(RSD=1.24%, n=3), 78.11%(RSD=0.97%,n=3),79.46%(RSD=1.03%,n=3)and 32.18%(RSD=1.16%,n=3),respectively. CONCLUSIONS:Using chito-san as clarifying agent shows good purification effect for Sanhuang yishen formula,which is simple. The optimized technology is stable and feasible.关键词
三黄益肾方/纯化工艺/正交试验/黄芪甲苷/总多糖/毛蕊异黄酮葡萄糖苷/壳聚糖/澄清剂Key words
Sanhuang yishen formula/Purification technology/Orthogonal test/Astragaloside/Total polysaccharide/Calyco-sin glucoside/Chitosan/Clarifying agent分类
医药卫生引用本文复制引用
吴小斌,王洛临,李洁环,钟如帆,袁柳萍,郭鸣..三黄益肾方的纯化工艺研究[J].中国药房,2017,28(7):957-960,4.基金项目
广东省科技计划项目(No.2014A020210017) （No.2014A020210017）
