中国药房2017，Vol.28Issue(7)：964-966,3.DOI:10.6039/j.issn.1001-0408.2017.07.29
正交试验优化白术配方颗粒的索氏提取工艺
Optimization of the Soxhlet Extraction Technology of Atractylodes macrocephala Formula Granules by Or-thogonal Test
摘要
Abstract
OBJECTIVE:To optimize the soxhlet extraction technology of Atractylodis macrocephalae,and to provide evi-dence for research and preparation of its formula granules. METHODS:Using the contents of atractylenolide Ⅰ,Ⅱ,Ⅲ as index, based on single factor test,the Soxhlet extraction technology of A. macrocephalae formula granules was optimized and verified by L9(34)orthogonal test with extraction time,solid-liquid ratio,extraction times as factors,and then compared with other technolo-gies (normal temperature extraction method,ultrasonic extraction method,reflux extraction method). RESULTS:The optimal ex-traction technology was as follows as 6-fold ethanol,extracting for 3 times,lasting for 8 h. Results of validation test showed that the extraction amounts of atractylenolide were 0.769,0.752,0.781 mg/g (RSD=1.99%,n=3) for 3 times,which were higher than the extraction amounts of other 3 methods(0.683,0.489,0.693 mg/g). CONCLUSIONS:The optimized extraction technolo-gy possesses high extraction rates of atractylenolide Ⅰ,Ⅱ,Ⅲ,and can be used for the extraction of internal ether from A. macro-cephalae formula granules.关键词
白术/白术内酯/索氏提取/工艺优化/正交试验Key words
Atractylodis macrocephalae/Atractylenolide/Soxhlet extraction/Technology optimization/Orthogonal test分类
医药卫生引用本文复制引用
李功华,李洪玉,费莹,寿旦..正交试验优化白术配方颗粒的索氏提取工艺[J].中国药房,2017,28(7):964-966,3.基金项目
浙江省中医药科技计划项目(No.2016ZB008) （No.2016ZB008）
