中国药房2017，Vol.28Issue(8)：1049-1052,4.DOI:10.6039/j.issn.1001-0408.2017.08.11
LC-MS/MS法和EMIT法测定人血清中丙戊酸浓度的对比研究
Comparative Stndy on Concentration Determination of Valproic Acid in Human Serum by LC-MS/MS and EMIT
时海浪 1王海东 2宋兴发2
作者信息
- 1. 新疆生产建设兵团第二师焉耆医院药剂科,新疆库尔勒841100
- 2. 连云港市第一人民医院药学部,江苏连云港222002
- 折叠
摘要
Abstract
OBJECTIVE:To compare the difference in the concentration determination of valproie acid (VPA) in human serum by LC-MS/MS and EMIT.METHODS:Both LC-MS/MS and EMIT methods were applied to determine the serum concentration of VPA in 144 inpatients or outpatients.The paired t-test,Pearson correlation analysis,Bland-Altman deviation chart and other methods were used to evaluate the difference in the results of concentration determination.RESULTS:The results of LC-MS/MS method was pos itively correlated with that of EMIT method (r=0.924,P＜0.05);the regression equation of them was cEMIT=0.920 7cLC.MS/MS-1.114 4 (r=0.924).Average serum concentrations of VPA determined by LC-MS/MS and EMIT were (49.9 ± 21.2) and (54.9 ± 21.3) μg/mL,with statistical significance (P＜0.05).The serum concentration of VPA determined by EMIT was higher than that by LC-MS/MS 8.3 μg/mL,95％ confidence interval was (-13.6,18.7).CONCLUSIONS:The serum concentration of VPA determined by LC-MS/MS and EMIT have high correlation.But the determination results still have certain difference,it is suggested to use same method for long term monitering.关键词
丙戊酸/血药浓度/液相色谱-串联质谱法/酶放大免疫法/差异Key words
Valproic acid/Serum concentration/LC-MS/MS/EMIT/Difference分类
医药卫生引用本文复制引用
时海浪,王海东,宋兴发..LC-MS/MS法和EMIT法测定人血清中丙戊酸浓度的对比研究[J].中国药房,2017,28(8):1049-1052,4.
