中国医科大学学报2017，Vol.46Issue(1)：11-16,6.DOI:10.12007/j.issn.0258⁃4646.2017.01.003
小鼠SPINK5基因重组过表达腺病毒载体构建及其对特应性皮炎小鼠模型皮损疗效的研究
Construction of Recombinant Adenovirus Vector Expressing Mouse SPINK5 Gene and Its Curative Effect on Skin Lesions in Atopic Dermatitis Mice
摘要
Abstract
Objective To construct a recombinant adenovirus vector expressing mouse SPINK5 gene,and observe its curative effect on the skin lesions in atopic dermatitis mice model. Methods By recombining DNA technology,the sequence of mouse SPINK5 gene was cloned into adeno?virus shuttle plasmid. Then it was transformed into HEK 293 cells with the adenoviral backbone plasmid to obtain the recombinant adenovirus. A mouse model of atopic dermatitis was established by system and local sensitization of Balb/c mice with ovalbumin . The effect of recombinant adeno?virus on the lesions of atopic dermatitis mice model was observed. Results The SPINK5 over?expressing adenovirus vector and atopic dermatitis mice model were successfully constructed. After 2 weeks of adenovirus?mediated SPINK5 gene intracutaneous injection,the redness and edema of lesions of AD model mice were obvious relieved. The pathological detection indicated that epidermal thickness and prickle cell layer ,inflammatory cell infiltration significant decreased accompanied with the model blank control. Conclusion The adenovirus?mediated SPINK5 gene had signifi?cant therapeutic effect to the atopic dermatitis mice model ,which provided a laboratory basis of application of SPINK5 gene product to therapy atopic dermatitis.关键词
SPINK5基因/重组腺病毒载体/特应性皮炎/小鼠模型Key words
SPINK5 gene/recombinant adenovirus vector/atopic dermatitis/mice model分类
医药卫生引用本文复制引用
狄正鸿,许静,侯殿东,纪莲,刘晓梅,孙鲁宁..小鼠SPINK5基因重组过表达腺病毒载体构建及其对特应性皮炎小鼠模型皮损疗效的研究[J].中国医科大学学报,2017,46(1):11-16,6.基金项目
国家自然科学基金(81301366) （81301366）
辽宁省自然科学基金(2013021004,2014021056) （2013021004,2014021056）
