中国医科大学学报2017，Vol.46Issue(1)：17-22,27,7.DOI:10.12007/j.issn.0258⁃4646.2017.01.004
Tubastatin A Hcl对慢性支气管哮喘小鼠的治疗作用
Therapeutic Effects of Tubastatin A Hcl in Chronic Murine Asthma Model
摘要
Abstract
Objective To investigate the effects of Tubastatin A Hcl,a selective HDAC6 inhibitor,on the development of chronic asthmatic mice with airway inflammation,airway remodeling and airway hyperresponsiveness. Methods BALB/C mice were randomly divided into control group, asthma group,dexamethasone group and Tubastatin A Hcl group. The airway resistance,total cells and different cells in BALF,IL?4,IL?5,TGF?β1 were detected by ELISA. HE、AB?PAS and Masson trichrome staining were carried out to assess the airway inflammation and remodeling. Immuno?histochemical staining and western blotting were adopted to determine the expression ofα?SMA and TGF?β1. Results After drugs treatment,air?way resistance decreased,and levels of IL?4,IL?5,TGF?β1,total inflammatory cells and eosinophils in BALF were relieved. Meanwhile,inflamma?tory cells infiltration,goblet cells metaplasia and collagen deposition in lung tissue were also reduced,but all of above the effects of dexamethasone were better than Tubastatin A Hcl. The expression ofα?SMA and TGF?β1 in the lung tissue decreased significantly after treatment ,in which Tu?bastatin A Hcl were slightly better than dexamethasone treatment. Conclusion Tubastatin A Hcl can effectively relieve airway inflammation ,air?way remodeling and airway hyperresponsiveness in chronic asthmatic mice ,but its effect of anti?inflammatory is worse than dexamethasone treat?ment,while it is better than dexamethasone in the effect of relief airway remodeling.关键词
哮喘/组蛋白去乙酰化酶/气道炎症/气道重塑/气道高反应Key words
asthma/histone deacetylase/airway inflammation/airway remodeling/airway hyperresponsiveness分类
医药卫生引用本文复制引用
任媛,苏新明,李孟露,赵萱,康健..Tubastatin A Hcl对慢性支气管哮喘小鼠的治疗作用[J].中国医科大学学报,2017,46(1):17-22,27,7.基金项目
国家自然科学基金(81300024) （81300024）
辽宁省科学技术计划(2013225049) （2013225049）
辽宁省教育厅创新团队(LT2013015) （LT2013015）
