中国医科大学学报2017，Vol.46Issue(2)：131-135,5.DOI:10.12007/j.issn.0258-4646.2017.02.008
褪黑素通过下调细胞周期蛋白及其激酶的表达抑制人骨肉瘤MG-63细胞增殖
Melatonin Inhibits the Proliferation of Human MG-63 Osteosarcoma Cells via Downregulation of Cyclins and CDKs
摘要
Abstract
Objective To investigate the potential mechanism that melatonin at higher concentrations inhibits the proliferation of human MG-63 osteosarcoma cells,so as to provide a certain experimental basis for the better application of melatonin in the treatment of diseases in Department of Orthopedics. Methods MG-63 cells cultured in vitro were treated with melatonin at a concentration of 4 mmol/L . Western blotting and real-time PCR method were used to analyze the effect of melatonin on the expression of cyclins and CDKs at protein and mRNA levels ,respectively. Results Western blotting and real-time PCR analyses showed that melatonin's inhibitory effect was possibly through the downregulation of cyclin D1 and CDK4 that related to the G1 phase,and downregulation of cyclin B1 and CDK1 that related to the G2/M phase. However,there was no obvious dif-ference of cyclin E,CDK2,and cyclin A,which were related to G1/S transition and S phase. Conclusion Melatonin may significantly inhibit hu-man osteosarcoma cell proliferation by inducing cell cycle arrest in a time-dependent manner,which is related to the downregulation of cyclin D1, CDK4,cyclin B1 and CDK1.关键词
褪黑素/骨肉瘤/细胞周期蛋白/细胞周期蛋白依赖性激酶Key words
melatonin/osteosarcoma/cyclins/cyclin-dependent kinase分类
医药卫生引用本文复制引用
刘立峰,潘玉涛,陈涤,李侠,刘养洲,普星宇,李增春..褪黑素通过下调细胞周期蛋白及其激酶的表达抑制人骨肉瘤MG-63细胞增殖[J].中国医科大学学报,2017,46(2):131-135,5.基金项目
上海市浦东新区卫生系统优秀青年医学人才培养(PWRq2013-15) （PWRq2013-15）
上海市卫生局面上项目(20134212) （20134212）
同济大学青年优秀人才培养行动计划(1507219028) （1507219028）
