中国医科大学学报2017，Vol.46Issue(2)：140-144,5.DOI:10.12007/j.issn.0258-4646.2017.02.010
5-Aza-CdR对DU145前列腺癌细胞系RNF180基因去甲基化作用的初步研究
Preliminary Study on the Effect of 5-Aza-CdR on the Demethylation of RNF180 in Prostate Cancer Cell Line DU145
王海光 1姜华茂 1左知润 1龙湟哲 1袁观远 1贾凡振1
作者信息
- 1. 锦州医科大学附属第一医院泌尿外科,辽宁 锦州 121001
- 折叠
摘要
Abstract
Objective To investigate the mechanism and cause of the inactivation of tumor suppressor gene RNF180 in prostate cancer cell line by observing the effect of 5-Aza-CdR on the RNF180 gene in prostate cancer cell line DU145. Methods MTT method was adopted to study the effect of 5-Aza-CdR(0,1,2,5,10,15 and 20μmoI/L)on the proliferation of prostate cancer cells. Western blotting,real-time PCR,and methyla-tion specific PCR(MSP)were separately used to detect the expression of RNF180 in prostate cancer cells before and after the treatment of the most suitable drug concentration(5μmoI/L). Results In a certain range,the effect of 5-Aza-CdR on the proliferation of prostate cancer cell line DU145 was increased with the increase of drug concentration and the time of drug treatment(P<0.05). After the treatment of the most suitable drug concentration,the protein and mRNA expression of RNF180 in prostate cancer cells was significantly increased(P<0.05),but the methyla-tion of the promoter region was obviously decreased. Conclusion 5-Aza-CdR can reverse the methylation status of RNF180 gene in DU145 pros-tate cancer cell line,and relieve the silencing status of RNF180gene expression.关键词
5-Aza-CdR/前列腺癌/RNF180/去甲基化Key words
5-Aza-CdR/prostate cancer/RNF180/demethylation分类
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王海光,姜华茂,左知润,龙湟哲,袁观远,贾凡振..5-Aza-CdR对DU145前列腺癌细胞系RNF180基因去甲基化作用的初步研究[J].中国医科大学学报,2017,46(2):140-144,5.
