中国医科大学学报2017，Vol.46Issue(2)：145-148,4.DOI:10.12007/j.issn.0258-4646.2017.02.011
NF-κB/p65 siRNA对小鼠Lewis肺癌移植瘤细胞凋亡和Bax表达的影响
Effects of NF-κB/p65 siRNA on Apoptosis and Bax Expression in Lung Tumour Cell Xenografts of Nude Mouse
曲耘 1吴荣 1张晓晔2
作者信息
- 1. 中国医科大学附属盛京医院 第二肿瘤科,沈阳 110004
- 2. 中国医科大学附属盛京医院 第四肿瘤科,沈阳 110004
- 折叠
摘要
Abstract
Objective To investigate the effects of nuclear factor-kappaB(NF-κB)/p65 signaling transduction on the apoptosis and expressions of apoptosis-related genes Bax in nude mouse lung tumour cell xenografts. Methods The nude mice Lewis lung carcinoma cell xenograft model was established,and then the mice were intraperitoneally injected with NF-κB/p65 small interfering RNA(siRNA). The apoptosis of xenografted tumor cells in nude mice was detected by TUNEL assay. Expressions of Bax mRNA and protein were detected by RT-PCR and Western blotting. Results The result of TUNEL assay demonstrated that p65 siRNA evidently evoked cell apoptosis. Compared to the PBS treatment group or the normal control mice,both mRNA and protein expression levels of Bax in tumor xenografts were significantly up-regulated. There were significant differences among three groups(P<0.05). Conclusion NF-κB/p65 subunit may play an essential role in cell apoptosis of Lewis lung tumor.关键词
NF-κB/p65/Lewis肺癌/凋亡/siRNA/移植瘤/BaxKey words
NF-κB/p65/Lewis lung cancer/apoptosis/small interfering RNA/xenografts/Bax分类
医药卫生引用本文复制引用
曲耘,吴荣,张晓晔..NF-κB/p65 siRNA对小鼠Lewis肺癌移植瘤细胞凋亡和Bax表达的影响[J].中国医科大学学报,2017,46(2):145-148,4.
