中国医科大学学报2017，Vol.46Issue(3)：193-196,4.DOI:10.12007/j.issn.0258-4646.2017.03.001
氯胺酮长期滥用后小鼠海马区caspase-3和PSD-95的表达变化
Change of Caspase-3 and PSD-95 Expression in Hippocampus of Mice with Chronic Ketamine Addiction
摘要
Abstract
Objective To study the changes of hippocampal caspase-3 and PSD-95 expression levels in the mice exposed to ketamine 30 mg/(kg·d)for three months. Methods Forty C57BL/6 mice were randomly divided into two groups,and the chronic ketamine addiction model was established by giving mice a three month course of daily intraperitoneal injections of ketamine. Immunohistochemical study and Western blot-ting were applied to observe the expression of caspase-3 and PSD-95 protein. Results There were more expression of caspase-3 and less of PSD-95 in ketamine group as detected by immuohistochemistry. Western blotting results showed caspase-3 active fragment level significantly increased com-pared to saline group,but PSD-95 protein level was decreased. Conclusion The increased level of caspase-3 protein and reduced expression of PSD-95 are observed after long-term ketamine administration. These findings may provide an evidence for the neurotoxicity in mouse hippocampus of chronic ketamine addition as a recreational drug.关键词
氯胺酮/半胱氨酸蛋白酶3/突触后密度蛋白95/海马Key words
ketamine/caspase-3/postsynaptic density 95/hippocampus分类
医药卫生引用本文复制引用
杜傲,吴旭,任鹏,何柏林,杨顺成,丁润涛,沈瑞鹏,李延柠,董志斌,卢岩..氯胺酮长期滥用后小鼠海马区caspase-3和PSD-95的表达变化[J].中国医科大学学报,2017,46(3):193-196,4.基金项目
国家自然科学基金(81171032,81100807,81671867) （81171032,81100807,81671867）
辽宁省教育厅科学研究一般项目(L2014316) （L2014316）
辽宁省科学技术基金(2015020514) （2015020514）
